Evidence map›Paper›PMID 37624265›Full record

ArticleToxins2023

A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in

Jessie Vandierendonck, Yana Girardin, Pieter De Bruyn, Henri De Greve, Remy Loris

Open access · goldAbstract read
In one paragraph

Article in Toxins, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. De novo gene synthesis by an antiviral reverse transcriptase.bioRxiv : the preprint server for biology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jessie VandierendonckCenter for Structural Biology, Vlaams Instituut voor Biotechnologie and Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
Yana GirardinCenter for Structural Biology, Vlaams Instituut voor Biotechnologie and Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.ORCID 0000-0001-5765-5794
Pieter De BruynCenter for Structural Biology, Vlaams Instituut voor Biotechnologie and Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
Henri De GreveCenter for Structural Biology, Vlaams Instituut voor Biotechnologie and Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
Remy LorisCenter for Structural Biology, Vlaams Instituut voor Biotechnologie and Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.ORCID 0000-0002-8862-3338
Vrije Universiteit Brussel · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Molecular cloning and controlled expression remain challenging when the target gene encodes a protein that is toxic to the host. We developed a set of multi-layer control systems to enable cloning of genes encoding proteins known to be highly toxic in

Indexed as

RiboswitchToxins, BiologicalCloning, MolecularDNA ReplicationEscherichia coliPromoter Regions, GeneticRiboswitchToxins, Biologicalcloningreplicational controlriboswitchtoxinstranscriptional controltranslational control

Identifiers

PMID37624265
PMCPMC10467106
OpenAlexW4385978711

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.