Evidence map›Paper›PMID 37624251›Full record

ArticleToxins2023

Purification and Activity of the Second Recombinant Enzyme for Biodegrading Linearized Microcystins by

Junhui Teng, Meijie Song, Qianqian Xu, Qianwen Zou, Haiyang Zhang, Chunhua Yin, Xiaolu Liu, Yang Liu, Hai Yan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxins, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Junhui TengSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Meijie SongSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Qianqian XuSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Qianwen ZouSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Haiyang ZhangSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.ORCID 0000-0002-2410-7078
Chunhua YinSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.ORCID 0000-0002-5067-2951
Xiaolu LiuSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.ORCID 0000-0003-4034-963X
Yang LiuSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Hai YanSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hepatotoxic microcystins (MCs) are produced and released by the harmful bloom-forming cyanobacteria, which severely threaten drinking water safety and human health due to their high toxicity, widespread distribution, and structural stability. The linearized microcystinase (MlrB) further hydrolyses the poisonous linearized MCs produced by the microcystinase-catalysed MCs to form tetrapeptides. Here, the purification and activity of MlrB were investigated. The results showed that the linearized products generated by 12.5 mg/L MC-LR and MC-RR were removed by purified recombinant MlrB at a protein concentration of 1 mg/L within 30 min. The high catalytic activity of MlrB can be obtained via heterologous expression and affinity purification, which lays the foundation for further studies on the properties and mechanism of MCs biodegradation enzymes.

Indexed as

MicrocystinsSphingomonadaceaeBiodegradation, EnvironmentalCatalysisChromatography, AffinityHumansMicrocystinsactivitybiodegradationlinearized microcystinasemicrocystinspurification

Identifiers

PMID37624251
PMCPMC10467064

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.