Evidence map›Paper›PMID 37624005›Full record

ArticlePathogens (Basel, Switzerland)2023

Molecular Detection and Identification of Piroplasm in Cattle from Kathmandu Valley, Nepal.

Medhavi Dhakal, Tulsi Ram Gompo, Prakash Devkota, Sharmila Chapagain Kafle, Janak Raj Subedi, Haiyan Gong, Hiroaki Arima, Richard Culleton, Masahito Asada, Kishor Pandey

Open access · goldAbstract read
In one paragraph

Article in Pathogens (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.4field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. From Benign Parasite to Emerging Pathogen: A Review ofAnimals : an open access journal from MDPI · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 3 countries.

Medhavi DhakalCentral Department of Zoology, Institute of Science and Technology, Tribhuvan University, Kathmandu 44601, Nepal.
Tulsi Ram GompoCentral Veterinary Laboratory, Kathmandu 44600, Nepal.ORCID 0000-0002-0973-0187
Prakash DevkotaCentral Veterinary Laboratory, Kathmandu 44600, Nepal.
Sharmila Chapagain KafleCentral Veterinary Laboratory, Kathmandu 44600, Nepal.
Janak Raj SubediCentral Department of Zoology, Institute of Science and Technology, Tribhuvan University, Kathmandu 44601, Nepal.
Haiyan GongShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
Hiroaki ArimaDepartment of International Health and Medical Anthropology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.ORCID 0000-0003-4713-5060
Richard CulletonDivision of Molecular Parasitology, Proteo-Science Centre, Ehime University, Ehime, Matsuyama 791-0295, Japan.ORCID 0000-0001-8808-5771
Masahito AsadaResearch Unit for Global Infection Control, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.ORCID 0000-0003-0042-6856
Kishor PandeyCentral Department of Zoology, Institute of Science and Technology, Tribhuvan University, Kathmandu 44601, Nepal.ORCID 0000-0002-0619-9108
Tribhuvan University · NPEhime University · JPNagasaki University · JPObihiro University of Agriculture and Veterinary Medicine · JPShanghai Veterinary Research Institute · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTick-borne protozoan parasites (TBPPs) cause significant problems for domestic animals' health in Nepal. TBPPs are routinely diagnosed by labor-intensive blood smear microscopy. In Nepal, there are some reports of

methodsOne hundred and six blood samples were collected from cattle in the Kathmandu Valley. Thin blood smears were prepared for microscopic examination. Parasite DNA was extracted from the blood, and nPCR and sequencing were performed to identify the TBPPs present.

resultsAmong the 106 samples, 45 (42.5%) were positive for piroplasm (

conclusionsTo the best of our knowledge, this study is the first to apply molecular detection and species determination of TBPPs in cattle in Nepal. The results of this study may be used as a starting point for the development of successful TBPP surveillance and prevention programs in Nepal.

Indexed as

BabesiacattleNepalPCRTheileria

Identifiers

PMID37624005
PMCPMC10458267
OpenAlexW4385835160

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.