Evidence map›Paper›PMID 37621534›Full record

ArticleVeterinary world2023

Expression of toll-like receptor 4 and its associated cytokines from peripheral blood mononuclear cells in Leghorn chickens.

Juthatip Jeenkeawpieam, Chananphat Tantikositruj, Warangkana Kitpipit, Anyarat Thiptara, Autchara Kayan, Kittichai Unjit, Siriluk Sintupachee, Chaiwat Boonkaewwan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary world, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Juthatip JeenkeawpieamAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Chananphat TantikositrujDepartment of Livestock Development, National Institute of Animal Health, Bangkok 10900, Thailand.
Warangkana KitpipitAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Anyarat ThiptaraDepartment of Livestock Development, Veterinary Research and Development Center (Upper Southern Region), Nakhon Si Thammarat 80110, Thailand.
Autchara KayanDepartment of Animal Science, Kasetsart University, Bangkok 10900, Thailand.
Kittichai UnjitDepartment of Livestock Development, National Institute of Animal Health, Bangkok 10900, Thailand.
Siriluk SintupacheeProgram in Creative Innovation in Science and Technology, Faculty of Science and Technology, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat, 80280, Thailand.
Chaiwat BoonkaewwanAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Walailak University · THDepartment of Physics, Mathematics and Informatics · BYDepartment of Livestock Development · THKasetsart University · THNakhon Si Thammarat Rajabhat University · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aim: Immune cells require toll-like receptor 4 (TLR4) to respond to lipopolysaccharides (LPS) by releasing pro-inflammatory cytokines. Peripheral blood mononuclear cells (PBMCs) are used to assess changes in cytokines released in response to diseases or pathogens. This study aimed to assess TLR4 gene expression in PBMCs from Leghorn chicken and the release of related cytokines. Materials and Methods: Peripheral blood mononuclear cells were isolated from blood samples obtained from Leghorn chicks. The PBMC cultures were stimulated with various concentrations of LPS (0.01-1 μg/ml). Polymerase chain reaction was used to detect TLR4 expression. The production of tumor necrosis factor-alpha (TNF-α) and interleukins (IL-1β and IL-6) was quantified using an enzyme-linked immunosorbent assay. Results: We found that TLR4 was expressed in both non-stimulated and stimulated Leghorn chicken PBMCs. In addition, the release of TNF-α, IL-1β, and IL-6 levels in Leghorn chicken PBMCs increased significantly with an increase in LPS concentration (0.01-1 μg/mL) (p < 0.05). Conclusion: Although TLR4 was expressed in both non-stimulated and stimulated Leghorn chicken PBMCs, its expression was significantly higher in LPS-stimulated PBMCs Therefore, the chicken's endotoxin response can be assessed by evaluating the pro-inflammatory cytokine production from PBMCs.

Indexed as

Leghorn chickenperipheral blood mononuclear cellpro-inflammatory cytokineToll-like receptor 4

Identifiers

PMID37621534
PMCPMC10446709
OpenAlexW4385502376

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.