Evidence map›Paper›PMID 37621395›Full record

ArticleFrontiers in microbiology2023

Evaluation and validation of laboratory procedures for the surveillance of ESBL-, AmpC-, and carbapenemase-producing

Rene S Hendriksen, Lina M Cavaco, Beatriz Guerra, Valeria Bortolaia, Yvonne Agersø, Christina Aaby Svendsen, Hanne Nørgaard Nielsen, Jette Sejer Kjeldgaard, Susanne Karlsmose Pedersen, Mette Fertner and 1 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Phenotypic and Genomic Profiling ofAntibiotics (Basel, Switzerland) · 2026
    Article
  3. Review
  4. Article
  5. Human-associated NDM-5-producing multidrug-resistantFrontiers in bioinformatics · 2026
    Article
  6. Article
  7. Article
  8. Phenotypic and Molecular Study of Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2025
    Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Rene S HendriksenNational Food Institute, Technical University of Denmark (DTU Food), European Union Reference Laboratory for Antimicrobial Resistance, Research Group for Global Capacity Building, Kgs. Lyngby, Denmark.
Lina M CavacoDepartment for Bacteria, Parasites and Fungi, Statens Serum Institut, Reference Laboratory for Antimicrobial Resistance, Copenhagen, Denmark.
Beatriz GuerraEuropean Food Safety Authority, Parma, Italy.
Valeria BortolaiaDepartment for Bacteria, Parasites and Fungi, Statens Serum Institut, Reference Laboratory for Antimicrobial Resistance, Copenhagen, Denmark.
Yvonne AgersøDepartment of Veterinary and Animal Sciences, University of Copenhagen University, Copenhagen, Denmark.
Christina Aaby SvendsenNational Food Institute, Technical University of Denmark (DTU Food), European Union Reference Laboratory for Antimicrobial Resistance, Research Group for Global Capacity Building, Kgs. Lyngby, Denmark.
Hanne Nørgaard NielsenNational Food Institute, Technical University of Denmark (DTU Food), European Union Reference Laboratory for Antimicrobial Resistance, Research Group for Global Capacity Building, Kgs. Lyngby, Denmark.
Jette Sejer KjeldgaardNational Food Institute, Technical University of Denmark (DTU Food), European Union Reference Laboratory for Antimicrobial Resistance, Research Group for Global Capacity Building, Kgs. Lyngby, Denmark.
Susanne Karlsmose PedersenNational Food Institute, Technical University of Denmark (DTU Food), European Union Reference Laboratory for Antimicrobial Resistance, Research Group for Global Capacity Building, Kgs. Lyngby, Denmark.
Mette FertnerNational Food Institute, Technical University of Denmark (DTU Food), European Union Reference Laboratory for Antimicrobial Resistance, Research Group for Global Capacity Building, Kgs. Lyngby, Denmark.
Henrik HasmanDepartment for Bacteria, Parasites and Fungi, Statens Serum Institut, Reference Laboratory for Antimicrobial Resistance, Copenhagen, Denmark.
Technical University of Denmark · DKStatens Serum Institut · DKEuropean Food Safety Authority · ITUniversity of Copenhagen · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Extended-spectrum β-lactamase- (ESBL) and AmpC- β-lactamase-producing Methods: Up to nine laboratory procedures were tested using minced meat as the matrix from beef, pork, and chicken as well as six procedures for the caecal content of cattle, pigs, and chicken. Variables included sample volume, pre-enrichment volume, pre-enrichment broth with and without antimicrobial supplementation, and incubation time/temperature. The procedures were evaluated against up to nine Results and discussion: The laboratory procedures tested revealed that the most sensitive and specific methodologies were based on a Buffered Peptone Water pre-enrichment of 225 ml to 25 g or 9 ml to 1 g for minced meat and caecal content, respectively, incubated at 37°C overnight, followed by inoculation onto MacConkey agar supplemented with 1 mg/L cefotaxime for detecting ESBL- and AmpC-producing

Indexed as

carbapenemaseEscherichia coliEuropean Unionextended-spectrum beta-lactamaseisolation methodprotocolsurveillance

Identifiers

PMID37621395
PMCPMC10445139
OpenAlexW4385703810

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.