Evidence map›Paper›PMID 37615890›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Cleavage Under Targets & Release Using Nuclease (CUT&RUN) of Histone Modifications in Epidermal Stem Cells of Adult Murine Skin.

Pooja Flora, Elena Ezhkova

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Pooja FloraBlack Family Stem Cell Institute, Department of Cell Development and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Elena EzhkovaBlack Family Stem Cell Institute, Department of Cell Development and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. elena.ezhkova@mssm.edu.
Icahn School of Medicine at Mount Sinai · US

Funding

Skin Biology and Diseases Resource-based Center at Mount SinaiP30AR079200 · NIAMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Elena Ezhkova · 2021 to 2026
$5.2M
Toward Understanding the Role of the Polycomb Complex in Skin ControlR01AR069078 · NIAMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI EZHKOVA, ELENA · 2016 to 2024
$4.4M
Toward understanding the molecular mechanisms of Merkel cell fate determinationR01AR063724 · NIAMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI EZHKOVA, ELENA · 2012 to 2021
$4.2M
Elucidating the role of Polycomb Repressive Complexes in Lingual Papillae DevelopmentR01DC017400 · NIDCD · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI EZHKOVA, ELENA · 2018 to 2022
$1.8M
Identifying epigenetic factors in control of epidermal stem cell longevity in the adult skinK99AR082956 · NIAMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI FLORA, POOJA · 2023 to 2024
$212k
NIAMS NIH HHS K99 AR082956NIAMS NIH HHS P30 AR079200NIAMS NIH HHS R01 AR063724NIAMS NIH HHS R01 AR069078NIDCD NIH HHS R01 DC017400
6 · The paper itself

Abstract

Cleavage Under Targets & Release Using Nuclease (CUT&RUN) has swiftly become the preferred procedure over the past few years for genomic mapping and detecting interactions between chromatin and its bound proteins. CUT&RUN is now being widely used for characterizing the epigenetic landscape in many cell types as it utilizes far less cell numbers when compared to Chromatin Immunoprecipitation-sequencing (ChIP-seq), thereby making it a powerful tool for researchers working with limited material. This protocol has been specifically optimized for detecting histone modifications in fluorescence-activated cell sorting (FACS)-isolated epidermal stem cells from adult mice.

Indexed as

ChromatinHistone CodeAnimalsEpigenomicsMiceProtein Processing, Post-TranslationalStem CellsChromatinCUT&RUNEpidermisEpigeneticsHistone modificationsStem cells

Identifiers

PMID37615890
PMCPMC10841116
OpenAlexW4386116794

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.