ArticleThe Journal of membrane biology1986
Parathyroid hormone-induced changes of the brush border topography and cytoskeleton in cultured renal proximal tubular cells.
Article in The Journal of membrane biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The trial behind it
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Who cites it
10 citing papers in PubMed, 50 citations in OpenAlex.
- Fluorescent ligand-directed co-localization of the parathyroid hormone 1 receptor with the brush-border scaffold complex of the proximal tubule reveals hormone-dependent changes in ezrin immunoreactivity consistent with inactivation.Biochimica et biophysica acta · 2012Article
- The parathyroid hormone 1 receptor directly binds to the FERM domain of ezrin, an interaction that supports apical receptor localization and signaling in LLC-PK1 cells.Molecular endocrinology (Baltimore, Md.) · 2009Article
- In vivo, villin is required for Ca(2+)-dependent F-actin disruption in intestinal brush borders.The Journal of cell biology · 1999Article
- Pathogenicity of the diffusely adhering strain Escherichia coli C1845: F1845 adhesin-decay accelerating factor interaction, brush border microvillus injury, and actin disassembly in cultured human intestinal epithelial cells.Infection and immunity · 1996Article
- Elevation of intracellular free calcium levels in HEp-2 cells infected with enteropathogenic Escherichia coli.Infection and immunity · 1991Article
- Protein phosphorylation by protein kinase C in HEp-2 cells infected with enteropathogenic Escherichia coli.Infection and immunity · 1990Article
- Seasonal effects on the daily variations of gentamicin-induced nephrotoxicity.Archives of toxicology · 1990Article
- Effects of parathyroid hormone on cytosolic free calcium concentration in individual rabbit connecting tubules.The Journal of clinical investigation · 1989Article
- Patch clamp study on primary culture of isolated proximal convoluted tubules.Pflugers Archiv : European journal of physiology · 1988Article
- Stimulation of inositol trisphosphate and diacylglycerol production in renal tubular cells by parathyroid hormone.The Journal of clinical investigation · 1987Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
In order to examine the possibility of parathyroid hormone-mediated ultrastructural rearrangements in target epithelium, isolated canine renal proximal tubular cells were grown on a collagen-coated semipermeable membrane in a defined medium. Scanning and transmission electron microscopy of these monolayers revealed abundant microvilli. Exposure of the proximal tubular cells to parathyroid hormone resulted in a biphasic changes involving: dramatic shortening and rarefaction of microvilli within 1 min; and recovery of microvillar topography after 5 min. A similar shortening of microvilli was observed following exposure to ionomycin, whereas incubation with cyclic AMP resulted in an elongation of microvilli. Parathyroid hormone stimulated cyclic AMP production and increased cytoplasmic free calcium concentration in cultured proximal tubular cells. Pretreatment of cells with a calmodulin inhibitor abolished the effect of parathyroid hormone on brush border topography. Shortening of microvilli was associated with a disappearance of microvillar core filaments. Staining of F-actin with fluoresceinphalloidin showed that parathyroid hormone resulted in fragmentation of stress fibers. It is concluded that parathyroid hormone-induced cell activation involves cytoplasmic-free calcium, calmodulin, and the cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.