ArticleCell2023
An antibiotic from an uncultured bacterium binds to an immutable target.
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Article in Cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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73citing papers in PubMed
19.1field-weighted citation impact, top 1% of its field
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Who cites it
73 citing papers in PubMed, 124 citations in OpenAlex.
- Natural molecule potentiates colistin efficacyVirulence · 2026Article
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- A double-layer plate method for rapid screening of cell wall-targeting antibiotic producers using a microbial whole-cell biosensor.Applied and environmental microbiology · 2026Article
- Antimicrobial peptides for tuberculosis in a post-antibiotic era: from in vitro promise to therapeutic potential.npj antimicrobials and resistance · 2026Review
- RECC: A Red/ET-CRISPR/Cas9-based system enabling genome mining of marineSynthetic and systems biotechnology · 2026Article
- Molecular action of NZ2114, a superior plectasin derivative.npj antimicrobials and resistance · 2026Article
- Bacterial cell envelope-targeting antibiotics.Nature reviews. Microbiology · 2026Review
- A target-guided drug repurposing strategy for antibacterial discovery.BMC microbiology · 2026Article
- Beyond the Single Isolate: Leveraging Plant-Associated Microbial Communities for Crop Resilience.Microorganisms · 2026Review
- Exploiting Pharmacokinetic/Pharmacodynamic Methods for Optimizing and Accelerating Drug Development of Innovative Anti-Infectives.ChemMedChem · 2026Review
- Genome-informed metabolomic re-analysis identifies serum-associated amino acid signatures in sepsis-associated bacterial pathogens.Frontiers in medicine · 2026Article
- An enhanced domestication method for uncultured bacteria.ISME communications · 2026Article
- The application of AI-driven and engineered intratumoral microbes in cancer therapy.Journal of translational medicine · 2025Review
- Antibiotic Resistance Crisis: From Bacterial Bioprospecting to Artificial Intelligence.Environmental microbiology reports · 2025Review
- A lignan compound regulates LPS modifications via PmrA/B signaling cascades to potentiate colistin efficacy in vivo.PLoS pathogens · 2025Article
- Identification of a Pair of Linear or Cyclic Naturally Inspired Bifunctional Lipopeptide Antibiotics That Overcome Antimicrobial Resistance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The last resort antibiotic daptomycin exhibits two independent antibacterial mechanisms of action.Nature communications · 2025Article
- Dynamic structures of a membrane transporter in native cellular membranes.Science advances · 2025Article
- YZ462 exhibits potent antibacterial activity against methicillin-resistant Staphylococcus aureus through bacterial membrane disruption.BMC microbiology · 2025Article
- The Role of Nucleotide Sequencing in Natural Product Drug Discovery.Journal of natural products · 2025Review
13 more citing papers are in PubMed but not listed here.
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Authors and funding
30 authors at 9 institutions in 5 countries.
Rhythm ShuklaNMR Spectroscopy, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands; Membrane Biochemistry and Biophysics, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
Aaron J PeoplesNovoBiotic Pharmaceuticals, Cambridge, MA 02138, USA.
Kevin C LudwigInstitute for Pharmaceutical Microbiology, University Hospital Bonn, University of Bonn, Bonn, Germany.
Sourav MaityMoleculaire Biofysica, Zernike Instituut, Rijksuniversiteit Groningen, Nijenborgh 4, 9747 AG Groningen, the Netherlands.
Maik G N DerksNMR Spectroscopy, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands; Membrane Biochemistry and Biophysics, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
Stefania De BenedettiInstitute for Pharmaceutical Microbiology, University Hospital Bonn, University of Bonn, Bonn, Germany.
Annika M KruegerMoleculaire Biofysica, Zernike Instituut, Rijksuniversiteit Groningen, Nijenborgh 4, 9747 AG Groningen, the Netherlands.
Bram J A VermeulenNMR Spectroscopy, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
Theresa HarbigIntegrative Transcriptomics, Center for Bioinformatics, University of Tübingen, 72070 Tübingen, Germany.
Francesca LavoreNMR Spectroscopy, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
Raj KumarNMR Spectroscopy, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
Rodrigo V HonoratoNMR Spectroscopy, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
Fabian GreinInstitute for Pharmaceutical Microbiology, University Hospital Bonn, University of Bonn, Bonn, Germany; German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany.
Kay NieseltIntegrative Transcriptomics, Center for Bioinformatics, University of Tübingen, 72070 Tübingen, Germany.
Yangping LiuThe Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
Alexandre M J J BonvinNMR Spectroscopy, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
Marc BaldusNMR Spectroscopy, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
Ulrich KubitscheckClausius-Institute for Physical and Theoretical Chemistry, University of Bonn, Bonn, Germany.
Eefjan BreukinkMembrane Biochemistry and Biophysics, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.
Catherine AchornNovoBiotic Pharmaceuticals, Cambridge, MA 02138, USA.
Anthony NittiNovoBiotic Pharmaceuticals, Cambridge, MA 02138, USA.
Christopher J SchwalenNovartis Institutes for Biomedical Research, Cambridge, MA 02139, USA.
Amy L SpoeringNovoBiotic Pharmaceuticals, Cambridge, MA 02138, USA.
Losee Lucy LingNovoBiotic Pharmaceuticals, Cambridge, MA 02138, USA.
Dallas HughesNovoBiotic Pharmaceuticals, Cambridge, MA 02138, USA.
Moreno LelliMagnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, via della Lastruccia 3, Sesto Fiorentino 50019, Italy; Consorzio Interuniversitario Risonanze Magnetiche MetalloProteine (CIRMMP), via Sacconi 6, Sesto Fiorentino 50019, Italy.
Wouter H RoosMoleculaire Biofysica, Zernike Instituut, Rijksuniversiteit Groningen, Nijenborgh 4, 9747 AG Groningen, the Netherlands.
Kim LewisAntimicrobial Discovery Center, Northeastern University, Department of Biology, Boston, MA 02115, USA.
Tanja SchneiderInstitute for Pharmaceutical Microbiology, University Hospital Bonn, University of Bonn, Bonn, Germany; German Center for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany. Electronic address: tschneider@uni-bonn.de.
Markus WeingarthNMR Spectroscopy, Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands. Electronic address: m.h.weingarth@uu.nl.
Utrecht University · NLNovoBiotic Pharmaceuticals · USUniversity of Bonn · DEUniversity of Groningen · NLUniversity of Tübingen · DEInteruniversity Consortium for Magnetic Resonance · ITNortheastern University · USNovartis (United States) · USTianjin Medical University · CN
Funding
Uncultured bacteria for the discovery of novel antimicrobialsP01AI118687 · NIAID · NORTHEASTERN UNIVERSITY · PI LEWIS, KIM · 2016 to 2020
$8.9MDiscovering antimicrobials acting against MDR pathogensR01AI170962 · NIAID · NORTHEASTERN UNIVERSITY · PI LEWIS, KIM · 2022 to 2025
$4.5MNIAID NIH HHS P01 AI118687NIAID NIH HHS R01 AI170962
Abstract
Antimicrobial resistance is a leading mortality factor worldwide. Here, we report the discovery of clovibactin, an antibiotic isolated from uncultured soil bacteria. Clovibactin efficiently kills drug-resistant Gram-positive bacterial pathogens without detectable resistance. Using biochemical assays, solid-state nuclear magnetic resonance, and atomic force microscopy, we dissect its mode of action. Clovibactin blocks cell wall synthesis by targeting pyrophosphate of multiple essential peptidoglycan precursors (C
Indexed as
Anti-Bacterial AgentsBacteriaSoil MicrobiologyBiological AssayDiphosphatesAnti-Bacterial AgentsDiphosphatesdiphosphoric acidanimal modelsantibiotic resistanceantibioticsatomic force microscopyautolysiscell wallinfectionlipid IImechanism of actionpeptidoglycansolid-state NMRuncultured bacteria
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.