Evidence map›Paper›PMID 37610586›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Anti-Nipah Virus Enzyme-Linked Immunosorbent Assays with Non-human Primate and Hamster Serum.

Elena Postnikova, Janie Liang, Shuiqing Yu, Yingyun Cai, Yu Cong, Michael R Holbrook

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
4.3field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Elena Postnikova *NIAID Integrated Research Facility, Ft. Detrick, Frederick, MD, USA.
Janie Liang *NIAID Integrated Research Facility, Ft. Detrick, Frederick, MD, USA.
Shuiqing YuNIAID Integrated Research Facility, Ft. Detrick, Frederick, MD, USA.
Yingyun CaiNIAID Integrated Research Facility, Ft. Detrick, Frederick, MD, USA.
Yu CongNIAID Integrated Research Facility, Ft. Detrick, Frederick, MD, USA.
Michael R HolbrookNIAID Integrated Research Facility, Ft. Detrick, Frederick, MD, USA. Michael.holbrook@nih.gov.
National Institutes of Health · US

Funding

NIAID NIH HHS HHSN272200700016I
6 · The paper itself

Abstract

Enzyme-linked Immunosorbent assays or ELISAs are a versatile method for detecting various immunological ligands of interest. As the name suggests, ELISAs rely on the interaction between a ligand and an antibody to produce results. In the study of infectious disease, ELISAs are commonly used to determine if a pathogen-specific immune response has occurred in a host organism. These assays can be performed in serosurveys as part of epidemiological investigations during, or following, an infectious disease outbreak. In the research environment, ELISAs are used to quantify the humoral immune response following infection or vaccination of a host organism. Data from these assays can be used to determine the type of immune response elicited (e.g. IgG1 vs IgG2) and the robustness of the response. Here, we describe ELISAs that were developed for the study of either hamsters or non-human primates vaccinated against Nipah virus infection, or infected with Nipah virus. The ELISAs described include assays for both IgG and IgM in the hamster and non-human primate models for Nipah virus-induced disease. An assay was also developed for the detection of IgA in bronchoalveolar lavage from non-human primates.

Indexed as

Biological AssayImmunoglobulin GAnimalsBronchoalveolar LavageCricetinaeEnzyme-Linked Immunosorbent AssayPrimatesImmunoglobulin GELISAHumoral responseIgA antibodyIgG antibodyIgM antibodyNipah virus

Identifiers

PMID37610586
OpenAlexW4386085010

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.