Evidence map›Paper›PMID 37610580›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

In Vivo Imaging of Nipah Virus Infection in Small Animal Rodent Models.

Kendra Johnson, Terry Juelich, Jennifer Smith, Benhur Lee, Alexander N Freiberg

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Kendra JohnsonDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
Terry JuelichDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
Jennifer SmithDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
Benhur LeeDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Alexander N FreibergDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA. anfreibe@utmb.edu.
The University of Texas Medical Branch at Galveston · USIcahn School of Medicine at Mount Sinai · US

Funding

Tropism, pathogenicity, and potential for zoonotic spillover of emergent henipa- and henipa-like virusesR01AI123449 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI LEE, BENHUR · 2016 to 2020
$2.7M
Bioavailable proteasome inhibitors as broad-spectrum antiviralsR33AI102267 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI FREIBERG, ALEXANDER NICLAS · 2014 to 2016
$1.2M
Microphysiologic human organ cultures to study Nipah virus-induced lung pathologyR21AI138233 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI FREIBERG, ALEXANDER NICLAS · 2018 to 2019
$434k
NIAID NIH HHS R01 AI123449NIAID NIH HHS R21 AI138233NIAID NIH HHS R33 AI102267
6 · The paper itself

Abstract

In vivo imaging system (IVIS) is a powerful tool for the study of infectious diseases, providing the ability to non-invasively follow viral infection in an individual animal over time. Recombinant henipaviruses expressing bioluminescent or fluorescent reporter proteins can be used both to monitor the spatial and temporal progression of Nipah virus (NiV) infection in vivo as well as in ex vivo tissues. Virally produced luciferases react with systemically administered substrate to produce bioluminescence that can then be detected via IVIS imaging, while fluorescent reporters inherently generate detectable fluorescence without a substrate. Here we describe protocols applying bioluminescent or fluorescent reporter expressing recombinant viruses to in vivo or ex vivo imaging of NiV infection.

Indexed as

Henipavirus InfectionsOrthopoxvirusAnimalsColoring AgentsDiagnostic ImagingModels, AnimalColoring AgentsFluorescenceIn vivo imagingLuminescenceRecombinant Nipah virusReporter genesRodent animal model

Identifiers

PMID37610580
OpenAlexW4386084608

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.