Evidence map›Paper›PMID 37610574›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Recombinant Cedar Virus: A Henipavirus Reverse Genetics Platform.

Moushimi Amaya, Christopher C Broder, Eric D Laing

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. AnFrontiers in chemical biology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Moushimi AmayaDepartment of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Christopher C BroderDepartment of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Eric D LaingDepartment of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA. eric.laing@usuhs.edu.

Funding

Project 3 - USUHSU19AI142764 · NIAID · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI ZEITLIN, LARRY · 2019 to 2023
$24.6M
Vaccines and Therapeutics for Nipah and Hendra virusU01AI077995 · NIAID · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI BRODER, CHRISTOPHER C · 2008 to 2012
$5.5M
Nipah Virus and Hendra Virus Entry and Virion AssemblyR01AI054715 · NIAID · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI BRODER, CHRISTOPHER C · 2006 to 2016
$3.1M
NIAID NIH HHS R01 AI054715NIAID NIH HHS U01 AI077995NIAID NIH HHS U19 AI142764
6 · The paper itself

Abstract

The isolation of Cedar virus, a nonpathogenic henipavirus that is closely related to the highly pathogenic Nipah virus and Hendra virus, provides a new platform for henipavirus experimentation and a tool to investigate biological differences among these viruses under less stringent biological containment. Here, we detail a reverse genetics system used to rescue two replication-competent, recombinant Cedar virus variants: a recombinant wild-type Cedar virus and a recombinant Cedar virus that express a green fluorescent protein from an open reading frame inserted between the phosphoprotein and matrix genes. This recombinant Cedar virus platform may be utilized to characterize the determinants of pathogenesis across the henipaviruses, investigate their receptor tropisms, and identify novel pan-henipavirus antivirals safely under biosafety level-2 conditions.

Indexed as

HenipavirusOrthopoxvirusAntiviral AgentsReverse GeneticsAntiviral AgentsCedar virusHenipavirusReverse geneticsVirus plaque assayVirus rescueVirus titration

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.