Evidence map›Paper›PMID 37608112›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Long-Range Polymerase Chain Reaction.

Ping Siu Kee, Harsheni Karunanathie, Simran D S Maggo, Martin A Kennedy, Eng Wee Chua

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Mechanisms of Antimicrobial Resistance inInfection and drug resistance · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ping Siu KeeDepartment of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand.
Harsheni KarunanathieCentre for Drug and Herbal Development, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Simran D S MaggoDepartment of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand.
Martin A KennedyDepartment of Pathology and Biomedical Science, University of Otago, Christchurch, New Zealand.
Eng Wee ChuaCentre for Drug and Herbal Development, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. cew85911@ukm.edu.my.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Polymerase chain reaction (PCR) is a laboratory technique used to amplify a targeted region of DNA, demarcated by a set of oligonucleotide primers. Long-range PCR is a form of PCR optimized to facilitate the amplification of large fragments. Using the adapted long-range PCR protocol described in this chapter, we were able to generate PCR products of 6.6, 7.2, 13, and 20 kb from human genomic DNA samples. For some of the long PCRs, successful amplification was not possible without the use of PCR enhancers. Thus, we also evaluated the impact of some enhancers on long-range PCR and included the findings as part of this updated chapter.

Indexed as

Polymerase Chain ReactionData CollectionDNA PrimersHumansDNA PrimersAgarose gel electrophoresisDNA polymeraseLong ampliconsLong-range polymerase chain reaction (PCR)PCR additivesPCR enhancersPharmacogeneticsPrimer designProofreading enzymeThermal cycling

Identifiers

PMID37608112

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.