Metaproteogenomic Profile of a Mesopelagic Adenylylsulfate Reductase: Course-Based Discovery Using the Ocean Protein Portal.

Madeline M Paoletti, Gregory P Fournier, Erin L Dolan, Mak A Saito

Open access · hybridAbstract read

In one paragraph

Article in Journal of proteome research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.2field-weighted citation impact, top 38% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Madeline M PaolettiDepartment of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

Gregory P FournierDepartment of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

Erin L DolanDepartment of Biochemistry and Molecular Biology, University of Georgia, B122 Life Sciences Bldg, Athens, Georgia 30602, United States.

Mak A SaitoDepartment of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, United States.ORCID 0000-0001-6040-9295

Massachusetts Institute of Technology · USUniversity of Georgia · USWoods Hole Oceanographic Institution · US

Funding

Response of the Bacterial Metalloproteome to Environmental ConditionsR01GM135709 · NIGMS · WOODS HOLE OCEANOGRAPHIC INSTITUTION · PI SAITO, MAKOTO · 2020 to 2023

$1.9M

NIGMS NIH HHS R01 GM135709

6 · The paper itself

Abstract

Adenylylsulfate reductase (Apr) is a flavoprotein with a dissimilatory sulfate reductase function. Its ability to catalyze the reverse reaction in sulfur oxidizers has propelled a complex phylogenetic history of transfers with sulfate reducers and made this enzyme an important protein in ocean sulfur cycling. As part of a graduate course, we analyzed metaproteomic data from the Ocean Protein Portal and observed evidence of Apr alpha (AprA) and beta (AprB) subunits in the Central Pacific Ocean. The protein was originally taxonomically attributed to

Indexed as

ProteomicsSulfatesOxidoreductases Acting on Sulfur Group DonorsPacific OceanPhylogenySulfuradenylylsulfate reductaseOxidoreductases Acting on Sulfur Group DonorsSulfatesSulfurbiogeochemistrycourse-based researchmetaproteogenomicsocean metaproteomics

Identifiers

PMID37607408

DOI10.1021/acs.jproteome.3c00152

PMCPMC10476264

OpenAlexW4386054555

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.