Evidence map›Paper›PMID 37606589›Full record

ArticleParasite (Paris, France)2023

Interactions between free-living amoebae and Cryptosporidium parvum: an experimental study.

Marion Lefebvre, Romy Razakandrainibe, Damien Schapman, Arnaud François, Damien Genty, Ludovic Galas, Isabelle Villena, Loic Favennec, Damien Costa

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite (Paris, France), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Marion LefebvreUniv Rouen Normandie, Laboratory of Parasitology-Mycology, EA7510 ESCAPE, University hospital of Rouen, Normandie, 76000 Rouen, France.ORCID 0000-0003-3283-0431
Romy RazakandrainibeUniv Rouen Normandie, Laboratory of Parasitology-Mycology, EA7510 ESCAPE, University hospital of Rouen, Normandie, 76000 Rouen, France - National Reference Center Cryptosporidiosis, microsporidia and other protozoa, University Hospital of Rouen, Normandie, 76000 Rouen, France.
Damien SchapmanUniv Rouen Normandie, INSERM, CNRS, HeRacLeS US 51 UAR 2026, PRIMACEN, 76000 Rouen, France.
Arnaud FrançoisUniv Rouen Normandie, Laboratory of Parasitology-Mycology, EA7510 ESCAPE, University hospital of Rouen, Normandie, 76000 Rouen, France - Department of anathomopathology, University Hospital of Rouen, Normandie, 76000 Rouen, France.
Damien GentyDepartment of anathomopathology, University Hospital of Rouen, Normandie, 76000 Rouen, France.
Ludovic GalasUniv Rouen Normandie, INSERM, CNRS, HeRacLeS US 51 UAR 2026, PRIMACEN, 76000 Rouen, France.ORCID 0000-0002-7374-0769
Isabelle VillenaReims Champagne-Ardenne University, Laboratory of Parasitology-Mycology, EA7510 ESCAPE, 51454 Reims, France.
Loic FavennecUniv Rouen Normandie, Laboratory of Parasitology-Mycology, EA7510 ESCAPE, University hospital of Rouen, Normandie, 76000 Rouen, France - National Reference Center Cryptosporidiosis, microsporidia and other protozoa, University Hospital of Rouen, Normandie, 76000 Rouen, France.ORCID 0000-0003-3386-2240
Damien CostaUniv Rouen Normandie, Laboratory of Parasitology-Mycology, EA7510 ESCAPE, University hospital of Rouen, Normandie, 76000 Rouen, France - National Reference Center Cryptosporidiosis, microsporidia and other protozoa, University Hospital of Rouen, Normandie, 76000 Rouen, France.
Université de Rouen Normandie · FRCentre National de la Recherche Scientifique · FREpidémiosurveillance et circulation des parasites dans les environnements · FRNormandie Université · FR

Funding

CHU - Université de Rouen
6 · The paper itself

Abstract

Free-Living Amebae (FLA) and Cryptosporidium oocysts occasionally share the same environment. From 2004 to 2016, Cryptosporidium was responsible for 60% of 905 worldwide waterborne outbreaks caused by protozoan parasites. The aim of this study was to evaluate interactions between C. parvum oocysts and two common FLAs (Acanthamoeba castellanii and Vermamoeba vermiformis) in a water environment. Encystment and survival of FLAs were evaluated by microscopy using trypan blue vital coloration. Oocysts were numerated on microscopy. Interactions were studied over time in conditions both unfavorable and favorable to phagocytosis. Potential phagocytosis was directly evaluated by several microscopic approaches and indirectly by numeration of microorganisms and oocyst infectivity evaluation. Occasional phagocytosis of C. parvum by FLAs was documented. However, oocyst concentrations did not decrease significantly, suggesting resistance of oocysts to phagocytosis. A temporary decrease of oocyst infectivity was observed in the presence of A. castellanii. The effect of these interactions on C. parvum infectivity is particularly interesting. The biofilm condition could favor the persistence or even the proliferation of oocysts over time. This study demonstrated interactions between C. parvum and FLAs. Further knowledge of the mechanisms involved in the decrease of oocyst infectivity in the presence of A. castellanii could facilitate the development of new therapeutic approaches.

Indexed as

AmoebaCryptosporidiosisCryptosporidiumCryptosporidium parvumAnimalsDisease OutbreaksOocystsBiofilmCryptosporidiumFree living amebaeInfectivityInteractionsPhagocytosis

Identifiers

PMID37606589
PMCPMC10443459
OpenAlexW4386046088

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.