ArticleAnalytical chemistry2023
Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber.
Article in Analytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Colloidal Light Scattering Distorts Fluorometric Quantification of mRNA in Lipid Nanoparticles.Current issues in molecular biology · 2026Article
- A sensitive and cost-effective "turn-on" aptasensor for carcinoembryonic antigen using Ce-UiO-66 as an ultraefficient nanoquencher.Mikrochimica acta · 2026Article
- Size- and Concentration-Resolved Detection of PET Microplastics in Real Water via Excitation-Emission Matrix Fluorescence Quenching of Polyamide-Derived Carbon Quantum Dots.Sensors (Basel, Switzerland) · 2026Article
- Novel immunosensor based on electrochemiluminescence inner filter effect and static quenching between fibrillary Ag-MOGs and SiOMikrochimica acta · 2024Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fluorescence attenuation caused by the absorption of the excitation and/or emission light is called the Inner Filter Effect (IFE) and can lead to a nonlinear fluorescence concentration response. In this article, we propose the AddAbs (Added Absorber) method, which counterintuitively corrects IFE by increasing the absorbance of the sample. In this method, an equal amount of a highly absorbing chromophore is added to each sample to compensate for the nonuniform quenching caused by different fluorophore concentrations. The AddAbs method was able to provide a linear fluorescence response (
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Registered trials
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