Evidence map›Paper›PMID 37604625›Full record

ArticleAnalytica chimica acta2023

A simple agglutination system for rapid antigen detection from large sample volumes with enhanced sensitivity.

Qin Wang, Nuttada Panpradist, Jack Henry Kotnik, Richard C Willson, Katerina Kourentzi, Zoe L Chau, Joanne K Liu, Barry R Lutz, James J Lai

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytica chimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Qin WangDepartment of Bioengineering, University of Washington, Seattle, WA, 98195-5061, USA.
Nuttada PanpradistDepartment of Bioengineering, University of Washington, Seattle, WA, 98195-5061, USA.
Jack Henry KotnikDepartment of Bioengineering, University of Washington, Seattle, WA, 98195-5061, USA.
Richard C WillsonDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, TX, 77204, USA.
Katerina KourentziDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, TX, 77204, USA.
Zoe L ChauDepartment of Bioengineering, University of Washington, Seattle, WA, 98195-5061, USA.
Joanne K LiuDepartment of Bioengineering, University of Washington, Seattle, WA, 98195-5061, USA.
Barry R LutzDepartment of Bioengineering, University of Washington, Seattle, WA, 98195-5061, USA. Electronic address: blutz@uw.edu.
James J LaiDepartment of Bioengineering, University of Washington, Seattle, WA, 98195-5061, USA; Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 106335, Taiwan. Electronic address: jilai@uw.edu.
University of Washington · USUniversity of Houston · USNational Taiwan University of Science and Technology · TW

Funding

HIV-specific target capture and quantitative isothermal amplification for acute HIV diagnosis and treatment monitoringR33AI140460 · NIAID · UNIVERSITY OF WASHINGTON · PI LUTZ, BARRY RYAN · 2021 to 2022
$1.5M
HIV-specific target capture and quantitative isothermal amplification for acute HIV diagnosis and treatment monitoringR61AI140460 · NIAID · UNIVERSITY OF WASHINGTON · PI LUTZ, BARRY RYAN · 2018 to 2020
$1.4M
DiagnosDisk - a highly sensitive point-of-care test for detecting SARS-CoV-2antigen in salivaR21AI163282 · NIAID · UNIVERSITY OF WASHINGTON · PI LAI, JAMES · 2021 to 2022
$367k
NIAID NIH HHS R21 AI163282NIAID NIH HHS R33 AI140460NIAID NIH HHS R61 AI140460
6 · The paper itself

Abstract

Lateral flow assays (LFAs) provide a simple and quick option for diagnosis and are widely adopted for point-of-care or at-home tests. However, their sensitivity is often limited. Most LFAs only allow 50 μL samples while various sample types such as saliva could be collected in much larger volumes. Adapting LFAs to accommodate larger sample volumes can improve assay sensitivity by increasing the number of target analytes available for detection. Here, a simple agglutination system comprising biotinylated antibody (Ab) and streptavidin (SA) is presented. The Ab and SA agglutinate into large aggregates due to multiple biotins per Ab and multiple biotin binding sites per SA. Dynamic light scattering (DLS) measurements showed that the agglutinated aggregate could reach a diameter of over 0.5 μm and over 1.5 μm using poly-SA. Through both experiments and Monte Carlo modeling, we found that high valency and equivalent concentrations of the two aggregating components were critical for successful agglutination. The simple agglutination system enables antigen capture from large sample volumes with biotinylated Ab and a swift transition into aggregates that can be collected via filtration. Combining the agglutination system with conventional immunoassays, an agglutination assay is proposed that enables antigen detection from large sample volumes using an in-house 3D-printed device. As a proof-of-concept, we developed an agglutination assay targeting SARS-CoV-2 nucleocapsid antigen for COVID-19 diagnosis from saliva. The assay showed a 10-fold sensitivity enhancement when increasing sample volume from 50 μL to 2 mL, with a final limit of detection (LoD) of 10 pg mL

Indexed as

COVID-19COVID-19 TestingAgglutinationAntibodiesBiotinHumansSARS-CoV-2AntibodiesBiotinAgglutinationAnalyte enrichmentCOVID-19 rapid antigen testLateral flow assaySalivaSensitivity enhancement

Identifiers

PMID37604625
PMCPMC10777812
OpenAlexW4385382938

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.