Evidence map›Paper›PMID 37603091›Full record

ArticleFunctional & integrative genomics2023

Cloning and functional analysis of the DXR gene and promoter region in Osmanthus fragrans var. semperflorens.

Zihan Wang, Taimoor Hassan Farooq, Hanjie He, Aitzaz A A Shahani, Rashida Hameed, Amna Fayyaz, Muhammad Sohail Yousaf, Jun Wang, Lili Chen

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Article in Functional & integrative genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed, 1 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors at 6 institutions in 2 countries.

Zihan WangCollege of Life Science and Technology, Central South University of Forestry and Technology, Hunan Changsha, 410004, China.
Taimoor Hassan FarooqCollege of Life Science and Technology, Central South University of Forestry and Technology, Hunan Changsha, 410004, China.
Hanjie HeCollege of Life Science and Technology, Central South University of Forestry and Technology, Hunan Changsha, 410004, China.
Aitzaz A A ShahaniKey Laboratory of Crop Sciences and Plant Breeding Genetics, College of Agriculture, Yanbian University, Yanji, Jilin, China.
Rashida HameedSchool of Environment and Safety Engineering, Institute of Environment and Ecology, Jiangsu University, Zhenjiang, China.
Amna FayyazDepartment of Plant Pathology, University of Davis, Davis, USA.
Muhammad Sohail YousafYale School of the Environment, New Haven, CT, 06511, USA.
Jun WangCollege of Life Science and Technology, Central South University of Forestry and Technology, Hunan Changsha, 410004, China. jwang0829@csuft.edu.cn.
Lili ChenCollege of Life Science and Technology, Central South University of Forestry and Technology, Hunan Changsha, 410004, China. cll185@163.com.
Central South University · CNCentral South University of Forestry and Technology · CNJiangsu University · CNUniversity of California, Davis · USYale University · USYanbian University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The precise biological function and activity of the deoxylulose-5-phosphate reductoisomerase (DXR) gene and its promoter in Osmanthus fragrans var. semperflorens remain unclear, even though OfDXR is known as the crucial enzyme involved in plant terpenoid synthesis. This study aimed to shed light on the role and activity of the OfDXR gene and its promoter in O. fragrans var. semperflorens by employing RACE-PCR and Hi-TAIL-PCR techniques for the cloning of the gene and promoter sequence from the petal tissue. Subsequently, genetic transformation and histochemical staining methods were utilized to analyze their function and activity. The OfDXR gene exhibited a DNA sequence length of 5241 bp, encompassing 12 exons and 11 introns. The corresponding cDNA sequence of the OfDXR gene was 1629 bp, encoding 474 amino acid residues. Expression analysis revealed that the OfDXR gene was predominantly active in the petals during the early full blooming stage. Overexpression of the OfDXR gene in Arabidopsis plants at the primary or full blooming stage led to an augmentation in the total terpenoid content. Furthermore, the promoter sequence of the OfDXR gene spanned a length of 1174 bp and contained conserved regulatory/response elements, demonstrating functional activity. These findings indicate that the OfDXR gene plays a pivotal role in terpenoid synthesis, while its promoter exhibits robust activity.

Indexed as

ArabidopsisPhosphatesCloning, MolecularIntronsPromoter Regions, GeneticTerpenesPhosphatesTerpenesDXRExpression analysisGenetic transformationHistochemical stainingOsmanthus fragransPromoter

Identifiers

PMID37603091
OpenAlexW4386019387

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.