ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2023
Gelatin Zymography Can Be Performed on Fixed Brain Tissue.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 3 citations in OpenAlex.
- Matrix metalloproteinase-2 as a novel regulator of glucose utilization by adipocytes.Scientific reports · 2025Article
- In Situ Zymography in Formalin-Fixed Paraffin-Embedded and Mineralized Tissues.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A Practical View of In-Gel Gelatin Zymography of Matrix Metalloproteinases 2 and 9 in Cardiac Tissue.Methods in molecular biology (Clifton, N.J.) · 2025Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gelatin zymography is widely used to detect gelatinase activity, which is performed on unfixed tissue because it is assumed that fixation inactivates enzymes. However, using fixed tissues has several advantages over using fresh tissues for such prevention of tissue decay, thereby preserving the proteins as well as the morphology and structure of the specimens. In this study, we investigated the effects of the four commonly used fixatives (ethanol, acetone, zinc-based fixative (ZBF), and paraformaldehyde (PFA)) on the gelatinolytic activity in mouse brain tissue. Multiple protocols were employed to extract proteins from the fixed brain tissue. Western blotting and
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