Evidence map›Paper›PMID 37598811›Full record

ArticleMethods (San Diego, Calif.)2023

Measuring occupancies of the nucleosome and nucleosome-interacting factors in vivo in Saccharomyces cerevisiae genome-wide.

Emily Biernat, Uzair Khan, Chhabi K Govind

Abstract read
In one paragraph

Article in Methods (San Diego, Calif.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Emily BiernatDepartment of Biological Sciences, Oakland University, Rochester, MI 48309, USA.
Uzair KhanDepartment of Biological Sciences, Oakland University, Rochester, MI 48309, USA.
Chhabi K GovindDepartment of Biological Sciences, Oakland University, Rochester, MI 48309, USA. Electronic address: govind@oakland.edu.

Funding

Roles for chromatin remodeler RSC and histone acetyltransferases in regulating chromatin structure and transcriptionR15GM148919 · NIGMS · OAKLAND UNIVERSITY · PI GOVIND, CHHABI K · 2022 to 2023
$492k
Role of histone chaperones in transcription and chromatin structureR15GM126449 · NIGMS · OAKLAND UNIVERSITY · PI GOVIND, CHHABI K · 2018 to 2018
$444k
NIGMS NIH HHS R15 GM126449NIGMS NIH HHS R15 GM148919
6 · The paper itself

Abstract

Nucleosomes are the repeating units of chromatin. The presence of nucleosomes poses a major impediment to all DNA-dependent processes. As a result, access to DNA in chromatin is dynamically regulated by many factors, including ATP-dependent chromatin remodeling complexes. Digestion of chromatin by micrococcal nuclease (MNase) followed by chromatin immunoprecipitation (ChIP) and sequencing can be leveraged to determine nucleosome occupancy, positioning, and the ability of chromatin interacting factors to alter chromatin accessibility. Here we describe the procedure for performing MNase and MNase ChIP-seq in detail.

Indexed as

NucleosomesSaccharomyces cerevisiaeChromatinChromatin ImmunoprecipitationChromatinNucleosomes

Identifiers

PMID37598811
PMCPMC10529416

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.