Evidence map›Paper›PMID 37597190›Full record

ArticleSTAR protocols2023

Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seq.

Isabel Cristina Vélez-Bermúdez, Shu-Jen Chou, Ai-Ping Chen, Wen-Dar Lin, Wolfgang Schmidt

Abstract read
In one paragraph

Article in STAR protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Isabel Cristina Vélez-BermúdezInstitute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan. Electronic address: icvb@gate.sinica.edu.tw.
Shu-Jen ChouInstitute of Plant and Microbial Biology, Genomic Technology Core, Academia Sinica, Taipei 11529, Taiwan.
Ai-Ping ChenInstitute of Plant and Microbial Biology, Genomic Technology Core, Academia Sinica, Taipei 11529, Taiwan.
Wen-Dar LinInstitute of Plant and Microbial Biology, Bioinformatics Core Lab, Academia Sinica, Taipei 11529, Taiwan.
Wolfgang SchmidtInstitute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan. Electronic address: wosh@gate.sinica.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ribosome profiling (Ribo-seq) measures ribosome density along messenger RNA (mRNA) transcripts and is used to estimate the "translational fitness" of a given mRNA in response to environmental or developmental cues with high resolution. Here, we describe a protocol for Ribo-seq in plants adapted for the model plant Arabidopsis thaliana. We describe steps for lysis and nucleolytic digestion and ribosome footprinting. We then detail library construction, sequencing, and data analysis.

Indexed as

ArabidopsisRibosome ProfilingProteomicsRibosomesRNA, MessengerRNA, MessengerGenomicsModel OrganismsMolecular BiologyPlant SciencesRNAseq

Identifiers

PMID37597190
PMCPMC10469065

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.