ArticlePlant physiology2023
Transcription factor module NLP-NIGT1 fine-tunes NITRATE TRANSPORTER2.1 expression.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- CsSPX-PHR/PHL-PHT1 and CsmiR399a-PHO2-PHT1/PHO1 Modules Orchestrate Pi Use Efficiency and Aluminium Tolerance in Tea Plants.Plant, cell & environment · 2026Article
- Genome-Wide Identification of NLP Family Genes in Cultivated Strawberry (International journal of molecular sciences · 2026Article
- Co-expression analysis identifies organ-specific gene regulatory networks responsive to phosphate limitation in hemp (Cannabis sativa L.).BMC plant biology · 2026Article
- Progress and new questions in understanding the mechanisms controlling plant nitrogen responses.Plant biotechnology (Tokyo, Japan) · 2026Article
- The Impact of Nitrogen and Phosphorus Interaction on Growth, Nutrient Absorption, and Signal Regulation in Woody Plants.Biology · 2025Article
- Genome-Wide Identification and Comprehensive Analysis of the GARP Transcription Factor Superfamily inGenes · 2025Article
- Down-regulation of the rice HRS1 HOMOLOG3 transcriptional repressor gene due to N deficiency directly co-activates ammonium and phosphate transporter genes.Journal of experimental botany · 2025Article
- A Microplate-Based Expression Monitoring System for ArabidopsisBio-protocol · 2024Article
- The clock-associated LUX ARRHYTHMO regulates high-affinity nitrate transport in Arabidopsis roots.The Plant journal : for cell and molecular biology · 2024Article
- MicroRNAs as potent regulators in nitrogen and phosphorus signaling transduction and their applications.Stress biology · 2024Review
- Role of GARP family transcription factors in the regulatory network for nitrogen and phosphorus acquisition.Journal of plant research · 2024Review
- Finding Balance in Adversity: Nitrate Signaling as the Key to Plant Growth, Resilience, and Stress Response.International journal of molecular sciences · 2023Review
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Authors and funding
2 authors at 2 institutions in 1 country.
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Abstract
Arabidopsis (Arabidopsis thaliana) high-affinity NITRATE TRANSPORTER2.1 (NRT2.1) plays a dominant role in the uptake of nitrate, the most important nitrogen (N) source for most terrestrial plants. The nitrate-inducible expression of NRT2.1 is regulated by NIN-LIKE PROTEIN (NLP) family transcriptional activators and NITRATE-INDUCIBLE GARP-TYPE TRANSCRIPTIONAL REPRESSOR1 (NIGT1) family transcriptional repressors. Phosphorus (P) availability also affects the expression of NRT2.1 because the PHOSPHATE STARVATION RESPONSE1 transcriptional activator activates NIGT1 genes in P-deficient environments. Here, we show a biology-based mathematical understanding of the complex regulation of NRT2.1 expression by multiple transcription factors using 2 different approaches: a microplate-based assay for the real-time measurement of temporal changes in NRT2.1 promoter activity under different nutritional conditions, and an ordinary differential equation (ODE)-based mathematical modeling of the NLP- and NIGT1-regulated expression patterns of NRT2.1. Both approaches consistently reveal that NIGT1 stabilizes the amplitude of NRT2.1 expression under a wide range of nitrate concentrations. Furthermore, the ODE model suggests that parameters such as the synthesis rate of NIGT1 mRNA and NIGT1 proteins and the affinity of NIGT1 proteins for the NRT2.1 promoter substantially influence the temporal expression patterns of NRT2.1 in response to nitrate. These results suggest that the NLP-NIGT1 feedforward loop allows a precise control of nitrate uptake. Hence, this study paves the way for understanding the complex regulation of nutrient acquisition in plants, thus facilitating engineered nutrient uptake and plant response patterns using synthetic biology approaches.
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