ArticlePLoS biology2023
A pathogen-specific isotope tracing approach reveals metabolic activities and fluxes of intracellular Salmonella.
Article in PLoS biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Identification of genes promoting fitness of a plant-associatedApplied and environmental microbiology · 2026Article
- Review
- Metabolic strategies of Enterobacteriaceae gut colonization.Nature reviews. Microbiology · 2026Review
- Host-microbiota-pathogen interactions drive Salmonella enterica serovar Typhimurium pathogenesis.Nature reviews. Microbiology · 2026Review
- How resistance-nodulation-division (RND) efflux pumps contribute to virulence.PLoS pathogens · 2026Review
- The master virulence regulator PhoP dictates carbon metabolism by controlling cyclic AMP synthesis in Salmonella.PLoS biology · 2025Article
- Deciphering novel enzymatic and non-enzymatic lysine lactylation inEmerging microbes & infections · 2025Article
- 13CFLUX - third-generation high-performance engine for isotopically (non)stationary 13C metabolic flux analysis.Bioinformatics (Oxford, England) · 2025Article
- Improved MALDI-MS Imaging of Polar andAnalytical chemistry · 2025Article
- Profiling Salmonella transcriptional dynamics during macrophage infection using a comprehensive reporter library.Nature microbiology · 2025Article
- Cytoplasmic MgProceedings of the National Academy of Sciences of the United States of America · 2025Article
- MtlD as a therapeutic target for intestinal and systemic bacterial infections.Journal of bacteriology · 2025Article
- Characterization and immunoprotective efficacy of a fumarate reductaseFrontiers in microbiology · 2025Article
- Succinate utilisation by Salmonella is inhibited by multiple regulatory systems.PLoS genetics · 2024Article
- Model validation and selection in metabolic flux analysis and flux balance analysis.Biotechnology progressReview
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Authors and funding
8 authors.
Funding
Abstract
Pathogenic bacteria proliferating inside mammalian host cells need to rapidly adapt to the intracellular environment. How they achieve this and scavenge essential nutrients from the host has been an open question due to the difficulties in distinguishing between bacterial and host metabolites in situ. Here, we capitalized on the inability of mammalian cells to metabolize mannitol to develop a stable isotopic labeling approach to track Salmonella enterica metabolites during intracellular proliferation in host macrophage and epithelial cells. By measuring label incorporation into Salmonella metabolites with liquid chromatography-mass spectrometry (LC-MS), and combining it with metabolic modeling, we identify relevant carbon sources used by Salmonella, uncover routes of their metabolization, and quantify relative reaction rates in central carbon metabolism. Our results underline the importance of the Entner-Doudoroff pathway (EDP) and the phosphoenolpyruvate carboxylase for intracellularly proliferating Salmonella. More broadly, our metabolic labeling strategy opens novel avenues for understanding the metabolism of pathogens inside host cells.
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