Evidence map›Paper›PMID 37594671›Full record

ArticleJournal of nephrology2023

Human parietal epithelial cells (PECs) and proteinuria in lupus nephritis: a role for ClC-5, megalin, and cubilin?

Monica Ceol, Lisa Gianesello, Hernan Trimarchi, Alberto Migliorini, Giovanna Priante, Claudia M Radu, Elena Naso, Annalisa Angelini, Lorenzo A Calò, Franca Anglani and 1 more

Open access · hybridAbstract read
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In one paragraph

Article in Journal of nephrology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Monica Ceol *Nephrology Unit- Kidney Histomorphology and Molecular Biology Laboratory, Department of Medicine-DIMED, University of Padua, Via Giustiniani, 2, 235128, Padua, Italy.
Lisa Gianesello *Nephrology Unit- Kidney Histomorphology and Molecular Biology Laboratory, Department of Medicine-DIMED, University of Padua, Via Giustiniani, 2, 235128, Padua, Italy.
Hernan TrimarchiNephrology Service, Hospital Británico de Buenos Aires, Buenos Aires, Argentina.
Alberto MiglioriniNephrology Unit- Kidney Histomorphology and Molecular Biology Laboratory, Department of Medicine-DIMED, University of Padua, Via Giustiniani, 2, 235128, Padua, Italy.
Giovanna PrianteNephrology Unit- Kidney Histomorphology and Molecular Biology Laboratory, Department of Medicine-DIMED, University of Padua, Via Giustiniani, 2, 235128, Padua, Italy.
Claudia M RaduGeneral Internal Medicine and Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine, University of Padua, Padua, Italy.
Elena NasoNephrology Unit- Kidney Histomorphology and Molecular Biology Laboratory, Department of Medicine-DIMED, University of Padua, Via Giustiniani, 2, 235128, Padua, Italy.
Annalisa AngeliniCardiovascular Pathology and Pathological Anatomy, Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padua, Padua, Italy.
Lorenzo A CalòNephrology Unit- Kidney Histomorphology and Molecular Biology Laboratory, Department of Medicine-DIMED, University of Padua, Via Giustiniani, 2, 235128, Padua, Italy.
Franca AnglaniNephrology Unit- Kidney Histomorphology and Molecular Biology Laboratory, Department of Medicine-DIMED, University of Padua, Via Giustiniani, 2, 235128, Padua, Italy.
Dorella Del PreteNephrology Unit- Kidney Histomorphology and Molecular Biology Laboratory, Department of Medicine-DIMED, University of Padua, Via Giustiniani, 2, 235128, Padua, Italy. dorella.delprete@unipd.it.ORCID 0000-0003-2141-3472
University of Padua · ITHospital Británico de Buenos Aires · AR

Funding

University of Padua CPDA157159
6 · The paper itself

Abstract

backgroundParietal epithelial cells are a heterogeneous population of cells located on Bowman's capsule. These cells are known to internalize albumin with a still undetermined mechanism, although albumin has been shown to induce phenotypic changes in parietal epithelial cells. Proximal tubular cells are the main actors in albumin handling via the macromolecular complex composed by ClC-5, megalin, and cubilin. This study investigated the role of ClC-5, megalin, and cubilin in the parietal epithelial cells of kidney biopsies from proteinuric lupus nephritis patients and control subjects and identified phenotypical changes occurring in the pathological milieu.

methodsImmunohistochemistry and immunofluorescence analyses for ClC-5, megalin, cubilin, ANXA3, podocalyxin, CD24, CD44, HSA, and LTA marker were performed on 23 kidney biopsies from patients with Lupus Nephritis and 9 control biopsies (obtained from nephrectomies for renal cancer).

resultsTwo sub-populations of hypertrophic parietal epithelial cells ANXA3

conclusionsOur results may provide further insight into the role of hypertrophic parietal epithelial cells located at the tubular pole and their possible involvement in protein endocytosis in lupus nephritis patients. These data also suggest that the presence of hypertrophic parietal epithelial cells in Bowman's capsule represents a potential resource for responding to protein overload observed in other glomerulonephritis.

Indexed as

Low Density Lipoprotein Receptor-Related Protein-2Lupus NephritisAlbuminsEpithelial CellsHumansKidney Tubules, ProximalProteinuriaReceptors, Cell SurfaceAlbuminsintrinsic factor-cobalamin receptorLow Density Lipoprotein Receptor-Related Protein-2Receptors, Cell SurfaceClC-5CubilinHypertrophic PECsKidney biopsyMegalin

Identifiers

PMID37594671
PMCPMC10703968
OpenAlexW4385969781

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.