Evidence map›Paper›PMID 37594613›Full record

ArticleStem cell reviews and reports2023

BMSC-exosomes miR-25-3p Regulates the p53 Signaling Pathway Through PTEN to Inhibit Cell Apoptosis and Ameliorate Liver Ischemia‒reperfusion Injury.

Hongnan Li, Weidong Lin, Guangzhi Zhang, Runsheng Liu, Minghai Qu, Jiayang Zhang, Xuekun Xing

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Article in Stem cell reviews and reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Hongnan LiDepartment of Public Health, Guilin Medical University, Zhiyuan Rd, Lingui District, Guilin, 541199, Guangxi, China.
Weidong LinSchool of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China.
Guangzhi ZhangInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Runsheng LiuDepartment of Public Health, Guilin Medical University, Zhiyuan Rd, Lingui District, Guilin, 541199, Guangxi, China.
Minghai QuDepartment of Public Health, Guilin Medical University, Zhiyuan Rd, Lingui District, Guilin, 541199, Guangxi, China.
Jiayang ZhangDepartment of Public Health, Guilin Medical University, Zhiyuan Rd, Lingui District, Guilin, 541199, Guangxi, China.
Xuekun XingDepartment of Public Health, Guilin Medical University, Zhiyuan Rd, Lingui District, Guilin, 541199, Guangxi, China. xuekun222@126.com.ORCID 0000-0003-0271-3699

Funding

Guangxi Science and Technology Base and Talent Special Project Guike AD20238094National Natural Science Foundation of China 82260802Youth Backbone Teachers Training Program of Henan Province 2019GGJS153
6 · The paper itself

Abstract

backgroundHepatic ischemia‒reperfusion injury (HIRI) is a pathological phenomenon during liver surgery, and bone marrow-mesenchymal stem cell (BMSC) exosomes (BMSC-Exos) regulate cell apoptosis and reduce ischemia‒reperfusion injury. We aimed to investigate the roles of BMSC-Exos and miR-25b-3p (enriched in BMSC-Exos) in HIRI and elucidate the underlying mechanisms. APPROACHES AND

resultsAn HIRI mouse model was constructed and preinjected with BMSC-Exos, agomir-miR-25, agomir-miR-NC, or PBS via the tail vein. Compared with mice with HIRI, mice with HIRI preinjected with BMSC-Exos had significantly decreased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and alleviated liver necrosis (P < 0.05). Quantitative hepatic transcriptomics showed that mice with HIRI preinjected with BMSC-Exos exhibited increased cell division, hematopoietic or lymphoid organ development and metabolic processes. miRNA sequencing of BMSC-Exos revealed that miR-25, which is related to I/R injury, was enriched in the exosomes. Compared with HIRI + NC mice, HIRI + miR-25b-3p mice had significantly increased miR-25b-3p expression, decreased ALT/AST levels and apoptosis-related protein expression (P < 0.05), and alleviated liver necrosis. The proliferation of AML-12 cells transfected with miR-25b-3p was significantly higher than that in the mimic NC group (P < 0.01) after hypoxia induction, and the apoptosis rate of cells was significantly lower than that in the NC group (P < 0.01). PTEN was identified as a miR-25b-3p target gene. PTEN expression was significantly diminished in miR-25b-3p-transfected AML12 cells (P < 0.05). HIRI + agomir-miR-25 mice displayed reduced PTEN expression and decreased p53 and cleaved caspase 3 levels compared to HIRI + NC mice.

conclusionsWe revealed the roles and underlying mechanisms of BMSC-Exos and miR-25 in HIRI, contributing to the prevention and treatment of HIRI.

Indexed as

ExosomesMesenchymal Stem CellsMicroRNAsReperfusion InjuryAnimalsApoptosisLiverMiceNecrosisSignal TransductionTumor Suppressor Protein p53MicroRNAsMIRN25 microRNA, mouseTumor Suppressor Protein p53ApoptosisBMSC ExosomesHIRImiR-25b-3pp53PTEN

Identifiers

PMID37594613

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.