Evidence map›Paper›PMID 37594542›Full record

ArticleArchives of virology2023

Poxvirus sensitivity of a novel diploid sheep embryonic heart cell line.

Halima Rhazi, Ikram Tifrouin, Karima Mikou, Oumaima Belayadi, Najete Safini, Meryem Alhyane, Khalid Omari Tadlaoui, Matthias Lenk, Mehdi Elharrak

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of virology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Halima RhaziLaboratory of Functional and Environmental Ecology, Faculty of Sciences and Technology Sidi Mohammed, Ben Abdellah University, BP 2202, Imouzzer Road, Fez, Morocco. rhazihalima.2020@gmail.com.ORCID http://orcid.org/0000-0002-7561-2429
Ikram TifrouinLaboratory of Research and Development Virology, MCI Animal Health, B.P: 278, Lot. 157, Zone Industrielle Sud-Ouest (ERAC), 28810, Mohammedia, Morocco.
Karima MikouLaboratory of Functional and Environmental Ecology, Faculty of Sciences and Technology Sidi Mohammed, Ben Abdellah University, BP 2202, Imouzzer Road, Fez, Morocco.
Oumaima BelayadiLaboratory of Research and Development Virology, MCI Animal Health, B.P: 278, Lot. 157, Zone Industrielle Sud-Ouest (ERAC), 28810, Mohammedia, Morocco.
Najete SafiniLaboratory of Research and Development Virology, MCI Animal Health, B.P: 278, Lot. 157, Zone Industrielle Sud-Ouest (ERAC), 28810, Mohammedia, Morocco.
Meryem AlhyaneLaboratory of Research and Development Virology, MCI Animal Health, B.P: 278, Lot. 157, Zone Industrielle Sud-Ouest (ERAC), 28810, Mohammedia, Morocco.
Khalid Omari TadlaouiLaboratory of Research and Development Virology, MCI Animal Health, B.P: 278, Lot. 157, Zone Industrielle Sud-Ouest (ERAC), 28810, Mohammedia, Morocco.
Matthias LenkDepartment of Experimental Animal Facilities and Biorisk Management, Collection of Cell Lines in Veterinary Medicine, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493, Greifswald, Insel Riems, Germany.
Mehdi ElharrakLaboratory of Research and Development Virology, MCI Animal Health, B.P: 278, Lot. 157, Zone Industrielle Sud-Ouest (ERAC), 28810, Mohammedia, Morocco.
M C I Santé Animale (Morocco) · MAFriedrich-Loeffler-Institut · DESidi Mohamed Ben Abdellah University · MA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lumpy skin disease virus (LSDV), camelpox virus (CPV), and orf virus (ORFV) are members of the family Poxviridae. These viruses are usually isolated or produced in embryonated eggs or primary cells because continuous cell lines are less sensitive to infection. Disadvantages of the use of eggs or primary cells include limited availability, potential endogenous contaminants, and a limited ability to perform multiple passages. In this study, we developed a diploid cell culture from sheep embryonic hearts (EHs) and demonstrated its high proliferative and long-term storage capacities. In addition, we demonstrated its sensitivity to representatives of three genera of the family Poxviridae: Capripoxvirus (LSDV), Orthopoxvirus (CPV), and Parapoxvirus (ORFV). The cell culture had a doubling time of 24 h and reached 40 passages with satisfactory yield. This is comparable to that observed in primary lamb testis (LT) cells at passage 5 (P5). After infection, each poxvirus titer was 7.0-7.6 log TCID

Indexed as

CapripoxvirusLumpy skin disease virusOrf virusPoxviridaeAnimalsCattleCell LineChlorocebus aethiopsDiploidyMaleSheepVero Cells

Identifiers

PMID37594542
OpenAlexW4385968778

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.