Evidence map›Paper›PMID 37594076›Full record

ArticleGenetics2023

Autophagy impairment and lifespan reduction caused by Atg1 RNAi or Atg18 RNAi expression in adult fruit flies (Drosophila melanogaster).

Mariah Bierlein, Joseph Charles, Trevor Polisuk-Balfour, Heidi Bretscher, Micaela Rice, Jacklyn Zvonar, Drake Pohl, Lindsey Winslow, Brennah Wasie, Sara Deurloo and 12 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Molecular mechanism on autophagy associated cardiovascular dysfunction inFrontiers in cell and developmental biology · 2025
    Review
  2. eLife · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 2 institutions in 1 country.

Mariah BierleinDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Joseph CharlesDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Trevor Polisuk-BalfourDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Heidi BretscherDepartment of Genetics, Cell Biology, and Developmental Biology, University of Minnesota, Minneapolis, MN 55455, USA.
Micaela RiceDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Jacklyn ZvonarDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Drake PohlDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Lindsey WinslowDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Brennah WasieDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Sara DeurlooDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Jordan Van WertDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Britney WilliamsDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Gabrielle AnkneyDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Zachary HarmonDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Erica DannDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Anna AzuzDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Alex Guzman-VargasDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Elizabeth KuhnsDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Thomas P NeufeldDepartment of Genetics, Cell Biology, and Developmental Biology, University of Minnesota, Minneapolis, MN 55455, USA.
Michael B O'ConnorDepartment of Genetics, Cell Biology, and Developmental Biology, University of Minnesota, Minneapolis, MN 55455, USA.
Felix AmissahSchool of Pharmacy, Ferris State University, Big Rapids, MI 49307, USA.
Changqi C ZhuDepartment of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA.
Ferris State University · USUniversity of Minnesota · US

Funding

Inter-organ signals regulating metabolism, physiology and developmental timingR35GM118029 · NIGMS · UNIVERSITY OF MINNESOTA · PI O'CONNOR, MICHAEL BRENDAN · 2016 to 2025
$3.8M
Mechanisms of TOR-dependent control of autophagy in DrosophilaR01GM136835 · NIGMS · UNIVERSITY OF MINNESOTA · PI NEUFELD, THOMAS P. · 2021 to 2024
$1.3M
Ferris State UniversityNIGMS NIH HHS R01 GM136835NIGMS NIH HHS R35 GM118029
6 · The paper itself

Abstract

Autophagy, an autophagosome and lysosome-based eukaryotic cellular degradation system, has previously been implicated in lifespan regulation in different animal models. In this report, we show that expression of the RNAi transgenes targeting the transcripts of the key autophagy genes Atg1 or Atg18 in adult fly muscle or glia does not affect the overall levels of autophagosomes in those tissues and does not change the lifespan of the tested flies but the lifespan reduction phenotype has become apparent when Atg1 RNAi or Atg18 RNAi is expressed ubiquitously in adult flies or after autophagy is eradicated through the knockdown of Atg1 or Atg18 in adult fly adipocytes. Lifespan reduction was also observed when Atg1 or Atg18 was knocked down in adult fly enteroblasts and midgut stem cells. Overexpression of wild-type Atg1 in adult fly muscle or adipocytes reduces the lifespan and causes accumulation of high levels of ubiquitinated protein aggregates in muscles. Our research data have highlighted the important functions of the key autophagy genes in adult fly adipocytes, enteroblasts, and midgut stem cells and their undetermined roles in adult fly muscle and glia for lifespan regulation.

Indexed as

AutophagyAutophagy-Related Protein-1 HomologDrosophila melanogasterDrosophila ProteinsLongevityAnimalsRNA InterferenceAtg1 protein, DrosophilaAutophagy-Related Protein-1 HomologDrosophila ProteinsagingAtg1Atg18Atg5Atg9autophagyDrosophila melanogasterfruit flylifespan

Identifiers

PMID37594076
PMCPMC11491525
OpenAlexW4385969008

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.