Evidence map›Paper›PMID 37592709›Full record

ArticleCell proliferation2024

NELFA and BCL2 induce the 2C-like state in mouse embryonic stem cells in a chemically defined medium.

Baojiang Wu, Yanqiu Wang, Xinhua Wei, Jingcheng Zhang, Jiahui Wu, Guifang Cao, Yong Zhang, Jun Liu, Xihe Li, Siqin Bao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Baojiang WuThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, China.ORCID https://orcid.org/0000-0002-8224-6885
Yanqiu WangThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, China.
Xinhua WeiThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, China.
Jingcheng ZhangKey Laboratory of Animal Biotechnology of the Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
Jiahui WuSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
Guifang CaoSchool of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
Yong ZhangKey Laboratory of Animal Biotechnology of the Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
Jun LiuKey Laboratory of Animal Biotechnology of the Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Northwest A&F University, Yangling, China.
Xihe LiThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, China.ORCID https://orcid.org/0000-0002-7001-7997
Siqin BaoThe State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, China.ORCID https://orcid.org/0000-0002-9582-3194
Inner Mongolia Agricultural University · CNMinistry of Agriculture and Rural Affairs · CN

Funding

Inner Mongolia Autonomous Region Natural Science Foundation 2021MS03003Inner Mongolia Engineering Technology Research Centre of Germplasm Resources Conservation and Utilization 21400-222526National Key Research and Development Program of China 2022YFD1302202/3National Natural Science Foundation of China 32060176National Training Program for College Students' Innovation 202210126037Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region NJYT23091Program of Advanced Foreign Experts Plan of Inner Mongolia Autonomous Region 21400-5213401Program of Higher-Level Talents of Inner Mongolia University 10000-21311201/058
6 · The paper itself

Abstract

A minority of mouse embryonic stem cells (ESCs) display totipotent features resembling 2-cell stage embryos and are known as 2-cell-like (2C-like) cells. However, how ESCs transit into this 2C-like state remains largely unknown. Here, we report that the overexpression of negative elongation factor A (Nelfa), a maternally provided factor, enhances the conversion of ESCs into 2C-like cells in chemically defined conditions, while the deletion of endogenous Nelfa does not block this transition. We also demonstrate that Nelfa overexpression significantly enhances somatic cell reprogramming efficiency. Interestingly, we found that the co-overexpression of Nelfa and Bcl2 robustly activates the 2C-like state in ESCs and endows the cells with dual cell fate potential. We further demonstrate that Bcl2 overexpression upregulates endogenous Nelfa expression and can induce the 2C-like state in ESCs even in the absence of Nelfa. Our findings highlight the importance of BCL2 in the regulation of the 2C-like state and provide insights into the mechanism underlying the roles of Nelfa and Bcl2 in the establishment and regulation of the totipotent state in mouse ESCs.

Indexed as

Embryonic Stem CellsMouse Embryonic Stem CellsAnimalsCell DifferentiationMiceProto-Oncogene Proteins c-bcl-2Proto-Oncogene Proteins c-bcl-2

Identifiers

PMID37592709
PMCPMC10849787
OpenAlexW4385969574

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.