ArticleBiotechnology for biofuels and bioproducts2023
Combinatorial engineering of betalain biosynthesis pathway in yeast Saccharomyces cerevisiae.
Article in Biotechnology for biofuels and bioproducts, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 26 citations in OpenAlex.
- Completing the acylation landscape in plants: GDSL enzymes join the BAHD and SCPL acyltransferase families.Journal of experimental botany · 2026Review
- Advances in anthocyanins: Chemical foundations, synthetic biology, molecular mechanisms, physicochemical properties, and applications in the food industry.Food chemistry: X · 2026Review
- Recombinant production of amaranthin and other betalain variants with yeast cell factories.Synthetic and systems biotechnology · 2025Article
- Coordinated synthesis of aromatic amino acid derived pigments in yeast via global transcriptional engineering.Microbial cell factories · 2025Article
- High-throughput metabolic engineering ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Screening of Plant UDP-Glycosyltransferases for Betanin Production in Yeast.Applied biochemistry and biotechnology · 2025Article
- Natural pigments derived from plants and microorganisms: classification, biosynthesis, and applications.Plant biotechnology journal · 2025Review
- BAHD acyltransferase from dragon fruit enables production of phyllocactin in engineered yeast.FEMS yeast research · 2025Article
- Identification of R2R3-MYB Transcription Factor Family Based onPlants (Basel, Switzerland) · 2025Article
- High-efficiency production of plant-derived pigment dopaxanthin in Escherichia coli by combination engineering.Microbial cell factories · 2024Article
- Beet red food colourant can be produced more sustainably with engineered Yarrowia lipolytica.Nature microbiology · 2023Article
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9 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundBetalains, comprising red-violet betacyanins and yellow-orange betaxanthins, are the hydrophilic vacuolar pigments that provide bright coloration to roots, fruits, and flowers of plants of the Caryophyllales order. Betanin extracted from red beets is permitted quantum satis as a natural red food colorant (E162). Due to antioxidant activity, betanin has potential health benefits.
resultsWe applied combinatorial engineering to find the optimal combination of a dozen tyrosine hydroxylase (TyH) and 4,5-dopa-estradiol-dioxygenase (DOD) variants. The best-engineered Saccharomyces cerevisiae strains produced over six-fold higher betaxanthins than previously reported. By genome-resequencing of these strains, we found out that two copies of DOD enzyme from Bougainvillea glabra together with TyH enzymes from Abronia nealleyi, Acleisanthes obtusa, and Cleretum bellidiforme were present in the three high-betaxanthin-producing isolates. Next, we expressed four variants of glucosyltransferases from Beta vulgaris for betanin biosynthesis. The highest titer of betanin (30.8 ± 0.14 mg/L after 48 h from 20 g/L glucose) was obtained when completing the biosynthesis pathway with UGT73A36 glucosyltransferase from Beta vulgaris. Finally, we investigated betalain transport in CEN.PK and S288C strains of Saccharomyces cerevisiae and identified a possible role of transporter genes QDR2 and APL1 in betanin transport.
conclusionsThis study shows the potential of combinatorial engineering of yeast cell factories for the biotechnological production of betanin.
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