Evidence map›Paper›PMID 37591901›Full record

ArticleScientific reports2023

Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure.

Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L Wolfson, Balpreet Singh Ahluwalia, Cynthia B Whitchurch, Louise Cole, Glen P Lockwood, Robin Diekmann and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Hong Mao *Vascular Biology Research Group, Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, The Arctic University of Norway, 9037, Tromsø, Norway. hong.mao@uit.no.
Karolina Szafranska *Vascular Biology Research Group, Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, The Arctic University of Norway, 9037, Tromsø, Norway. karolina.szafranska@uit.no.
Larissa KruseVascular Biology Research Group, Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, The Arctic University of Norway, 9037, Tromsø, Norway.
Christopher HolteVascular Biology Research Group, Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, The Arctic University of Norway, 9037, Tromsø, Norway.
Deanna L WolfsonOptical Nanoscopy Research Group, Department of Physics and Technology, Faculty of Science and Technology, University of Tromsø, The Arctic University of Norway, Tromsø, Norway.
Balpreet Singh AhluwaliaOptical Nanoscopy Research Group, Department of Physics and Technology, Faculty of Science and Technology, University of Tromsø, The Arctic University of Norway, Tromsø, Norway.
Cynthia B WhitchurchMicrobial Imaging Facility, The ithree Institute, University of Technology Sydney, Ultimo, NSW, Australia.
Louise ColeMicrobial Imaging Facility, The ithree Institute, University of Technology Sydney, Ultimo, NSW, Australia.
Glen P LockwoodCentre for Education and Research, ANZAC Research Institute, Concord Repatriation General Hospital, Concord, NSW, Australia.
Robin DiekmannCell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
David Le CouteurCentre for Education and Research, ANZAC Research Institute, Concord Repatriation General Hospital, Concord, NSW, Australia.
Victoria C CoggerCentre for Education and Research, ANZAC Research Institute, Concord Repatriation General Hospital, Concord, NSW, Australia.
Peter A G McCourtVascular Biology Research Group, Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, The Arctic University of Norway, 9037, Tromsø, Norway.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Xanthines such as caffeine and theobromine are among the most consumed psychoactive stimulants in the world, either as natural components of coffee, tea and chocolate, or as added ingredients. The present study assessed if xanthines affect liver sinusoidal endothelial cells (LSEC). Cultured primary rat LSEC were challenged with xanthines at concentrations typically obtained from normal consumption of xanthine-containing beverages, food or medicines; and at higher concentrations below the in vitro toxic limit. The fenestrated morphology of LSEC were examined with scanning electron and structured illumination microscopy. All xanthine challenges had no toxic effects on LSEC ultrastructure as judged by LSEC fenestration morphology, or function as determined by endocytosis studies. All xanthines in high concentrations (150 μg/mL) increased fenestration frequency but at physiologically relevant concentrations, only theobromine (8 μg/mL) showed an effect. LSEC porosity was influenced only by high caffeine doses which also shifted the fenestration distribution towards smaller pores. Moreover, a dose-dependent increase in fenestration number was observed after caffeine treatment. If these compounds induce similar changes in vivo, age-related reduction of LSEC porosity can be reversed by oral treatment with theobromine or with other xanthines using targeted delivery.

Indexed as

CaffeineTheobromineAnimalsEndothelial CellsLiverRatsXanthineCaffeineTheobromineXanthine

Identifiers

PMID37591901
PMCPMC10435486

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.