ArticleDevelopment (Cambridge, England)2023
Control of gastruloid patterning and morphogenesis by the Erk and Akt signaling pathways.
Article in Development (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Design and optimization of a kinase-controlled allosteric switch.Nature methods · 2026Article
- Retinoic acid and FGF signaling interact to control elongation and lineage specification in a mouse gastruloid model.Development (Cambridge, England) · 2026Article
- MAPK/ERK signaling regulates H3K9me3 heterochromatin reorganization to confer mesendoderm developmental competence.Nucleic acids research · 2026Article
- Pleiotropic germline PTEN mutations influence gastrulation through dysregulated AKT activation.NPJ genomic medicine · 2026Article
- Fine-tuning ERK activity enables proliferation-differentiation balance during lineage specification of human embryonic stem cells.PLoS biology · 2026Article
- Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids.Development (Cambridge, England) · 2026Article
- N2B27 media formulations influence gastruloid development.Development (Cambridge, England) · 2025Article
- Fine-tuning mechanical constraints reveals uncoupled patterning and gene expression programs in murine gastruloids.Development (Cambridge, England) · 2025Article
- Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids.bioRxiv : the preprint server for biology · 2025Article
- MAPK/ERK signaling blocks ectopic H3K9me3 heterochromatin formation to confer mesoderm and endoderm developmental competence.bioRxiv : the preprint server for biology · 2025Article
- Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudoembryos.Science advances · 2025Article
- Gastruloid patterning reflects division of labor among biased stem cell clones.bioRxiv : the preprint server for biology · 2025Article
- Progress in understanding the vertebrate segmentation clock.Nature reviews. Genetics · 2025Review
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
- Three-dimensional stem cell models of mammalian gastrulation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- In Vitro Models of Cardiovascular Disease: Embryoid Bodies, Organoids and Everything in Between.Biomedicines · 2024Review
- Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking.Nature cell biology · 2024Article
- Precise and scalable self-organization in mammalian pseudo-embryos.Nature structural & molecular biology · 2024Article
- Robust axis elongation by Nodal-dependent restriction of BMP signaling.Development (Cambridge, England) · 2024Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Many developmental processes rely on the localized activation of receptor tyrosine kinases and their canonical downstream effectors Erk and Akt, yet the specific roles played by each of these signals is still poorly understood. Gastruloids, 3D cell culture models of mammalian gastrulation and axial elongation, enable quantitative dissection of signaling patterns and cell responses in a simplified, experimentally accessible context. We find that mouse gastruloids contain posterior-to-anterior gradients of Erk and Akt phosphorylation induced by distinct receptor tyrosine kinases, with features of the Erk pattern and expression of its downstream target Snail exhibiting hallmarks of size-invariant scaling. Both Erk and Akt signaling contribute to cell proliferation, whereas Erk activation is also sufficient to induce Snail expression and precipitate profound tissue shape changes. We further uncover that Erk signaling is sufficient to convert the entire gastruloid to one of two mesodermal fates depending on position along the anteroposterior axis. In all, these data demonstrate functional roles for two core signaling gradients in mammalian development and suggest how these modules might be harnessed to engineer user-defined tissues with predictable shapes and cell fates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.