ArticleBiotechnology letters2023
Establishment of protoplasts transient expression system in Pinellia ternata (Thunb.) Breit.
Article in Biotechnology letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- Research Progress on Preparation, Transformation and Application of Protoplasts Derived from Medicinal Plants.Plants (Basel, Switzerland) · 2026Review
- ARGOS homolog evolution in brassicaceae and the role of BnaC6.ARGOS in conferring drought tolerance via expression-responsive localization and reduced seed porosity in rapeseed.BMC plant biology · 2025Article
- Establishment of efficient hypocotyl-derived protoplast isolation and its application in soybean (Frontiers in plant science · 2025Article
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Authors and funding
8 authors.
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Abstract
objectiveIn this study, we established an efficient and rapid transient expression system in the protoplasts of Pinellia ternata (Thunb.) Breit. (P. ternata).
resultsThe protoplasts of P. ternata were prepared from plant leaves as the source material by digesting them with the combination of 20 g·l
conclusionsWe present the method that can be applicable for studying both gene verification and expression in P. ternata protoplasts, thus allowing for engineering the improved varieties of P. ternata through molecular plant breeding techniques. This method can also be widely applicable for analyzing protein interactions, detecting promoter activity, for somatic cell fusion in plant breeding, as well as for other related studies.
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