Evidence map›Paper›PMID 37587825›Full record

ArticleCurrent molecular medicine2024

Exosomes derived from Mouse Bone Marrow Mesenchymal Stem Cells Attenuate Nucleus Pulposus Cell Apoptosis via the miR-155- 5p/Trim32 Axis.

Fei Chen, Shangze Li, Ji Wu, Qunfeng Guo, Haibin Wang, Bin Ni, Jun Yang

Abstract read
PubMed Publisher
In one paragraph

Article in Current molecular medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Fei ChenDepartment of Orthopaedics, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.
Shangze LiDepartment of Orthopaedics, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.
Ji WuDepartment of Orthopaedics, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.
Qunfeng GuoDepartment of Orthopaedics, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.
Haibin WangDepartment of Orthopaedics, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.
Bin NiDepartment of Orthopaedics, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.
Jun YangDepartment of Orthopaedics, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.

Funding

National Natural Science Foundation of China 82002336Science and Technology Commission of Shanghai Municipality 21ZR1478300
6 · The paper itself

Abstract

backgroundLower back pain, shown to be strongly associated with IVDD, affects approximately 60%-80% of adults and has a considerable societal and economic impact. Evidence suggests that IVDD, caused by abnormal apoptosis of nucleus pulposus cells (NPCs), can be treated using MSC-derived exosomes.

objectiveThis study aimed to evaluate the role of miR155-5p/Trim32 in intervertebral disc disease (IVDD) and elucidate the underlying molecular mechanisms. Deregulating miR-155 has been shown to promote Fas-mediated apoptosis in human IVDD. Evidence also suggests that tripartite motif (TRIM)-containing protein 32 (Trim32) is regulated by miR-155. However, the role of miR155-5p/Trim32 in IVDD remains unclear.

methodsCell viability was checked using CCK-8 kits, and flow cytometry was used to analyze cell cycle and apoptosis. Cell migration was measured with a Transwell assay, while a luciferase assay was adopted to study how miR-155-5p interacts with Trim32. The roles of Trim32 and miR-155-5p were studied by silencing or up-regulating them in NPCs, while qPCR and immunoblots were used to evaluate mRNA and protein changes, respectively.

resultsTNF-α treatment significantly inhibited cell viability but promoted Trim32 expression in primary mouse NPCs. Administration of bone marrow mesenchymal stem cells (BMSCs) attenuated primary NPC cell cycle arrest and apoptosis induced by TNF- α. BMSCs-derived exosomes could be taken up by NPCs to inhibit TNF-α-induced cell cycle arrest and apoptosis through miR-155-5p. Examination of the underlying mechanism showed that miR-155-5p targeted Trim32. Moreover, Trim32 overexpression inhibited the effect of BMSCs-derived exosomes on primary mouse NPC cell apoptosis induced by TNF-α.

conclusionOverall, these findings suggest that exosomes from BMSCs can suppress TNF-α-induced cell cycle arrest and apoptosis in primary mouse NPCs through the delivery of miR-155-5p by targeting Trim32. This study provides a promising therapeutic strategy for IVDD.

Indexed as

ApoptosisExosomesMesenchymal Stem CellsMicroRNAsNucleus PulposusAnimalsCell MovementCell ProliferationHumansIntervertebral Disc DegenerationMaleMiceTripartite Motif ProteinsUbiquitin-Protein LigasesMicroRNAsMirn155 microRNA, mouseTripartite Motif ProteinsUbiquitin-Protein Ligasescell deathexosomesIntervertebral disc degenerationmesenchymal stem cellsmiRNATrim32.

Identifiers

PMID37587825
OpenAlexW4385898023

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.