ArticleParasites & vectors2023
Comparative study of a broad qPCR panel and centrifugal flotation for detection of gastrointestinal parasites in fecal samples from dogs and cats in the United States.
Article in Parasites & vectors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 24 citations in OpenAlex.
- The detection of three independent benzimidazole resistance mutations in a single Ancylostoma caninum population from a Golden Retriever breeding kennel in Sao Paulo, Brazil.International journal for parasitology. Drugs and drug resistance · 2026Article
- Prevalence and distribution ofOne health (Amsterdam, Netherlands) · 2026Article
- Comparison of multilocus genotyping and a commercial beta-giardin qPCR assay for detection of Giardia duodenalis zoonotic assemblages in cat and dog samples.Parasites & vectors · 2026Article
- Efficacy evaluation of a new oral chewable tablet containing fluralaner, moxidectin, and pyrantel (BRAVECTOParasites & vectors · 2026Article
- High prevalence of gastrointestinal parasites in dogs from Saipan, Northern Mariana Islands, including the zoonotic Ancylostoma ceylanicum.Parasites & vectors · 2026Article
- Giardia duodenalis in domestic cats from the US and Canada: Prevalence and zoonotic potential.PloS one · 2026Article
- Local field study on the effectiveness and safety of milbemycin oxime and lotilaner (CredelioParasitology research · 2025Article
- Article
- Prevalence of Toxocara infection and associated risk factors: a cross-sectional study in Zhejiang, China.Infectious diseases of poverty · 2025Article
- Progress in serology and molecular biology of equine parasite diagnosis: sustainable control strategies.Frontiers in veterinary science · 2025Review
- First Characterization and Zoonotic Potential Evaluation ofTransboundary and emerging diseases · 2025Article
- Zoonotic Enteric Nematodes and Dermatophytes in Cat Cafés: An Investigation in the Bangkok Metropolitan Area, Thailand.Veterinary sciences · 2024Article
- Benzimidazole F167Y polymorphism in the canine hookworm, Ancylostoma caninum: Widespread geographic, seasonal, age, and breed distribution in United States and Canada dogs.International journal for parasitology. Drugs and drug resistance · 2024Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFor decades, zinc sulfate centrifugal fecal flotation microscopy (ZCF) has been the mainstay technique for gastrointestinal (GI) parasite screening at veterinary clinics and laboratories. Elsewhere, PCR has replaced microscopy because of generally increased sensitivity and detection capabilities; however, until recently it has been unavailable commercially. Therefore, the primary aim of this study was to compare the performance of real-time PCR (qPCR) and ZCF for fecal parasite screening. Secondary aims included further characterization of markers for hookworm treatment resistance and Giardia spp. assemblages with zoonotic potential and qPCR optimization.
methodsA convenience sampling of 931 canine/feline fecal samples submitted to a veterinary reference laboratory for routine ZCF from the Northeast US (11/2022) was subsequently evaluated by a broad qPCR panel following retention release. Detection frequency and agreement (kappa statistics) were evaluated between ZCF and qPCR for seven GI parasites [hookworm/(Ancylostoma spp.), roundworm/(Toxocara spp.), whipworm/(Trichuris spp.), Giardia duodenalis, Cystoisospora spp., Toxoplasma gondii, and Tritrichomonas blagburni] and detections per sample. Total detection frequencies were compared using a paired t-test; positive sample and co-infection frequencies were compared using Pearson's chi-squared test (p ≤ 0.05 significant) and qPCR frequency for hookworm benzimidazole (BZ) resistance (F167Y) and zoonotic Giardia spp. assemblage markers calculated. Confirmatory testing, characterization, and qPCR optimization were carried out with Sanger sequencing.
resultsqPCR detected a significantly higher overall parasite frequency (n = 679) compared to ZCF (n = 437) [p = < 0.0001, t = 14.38, degrees-of-freedom (df) = 930] and 2.6 × the co-infections [qPCR (n = 172) vs. ZCF (n = 66)], which was also significant (p = < 0.0001, X
conclusionsThese results demonstrate the statistically significant detection frequency advantage offered by qPCR compared to routine ZCF for both single and co-infections. While overall agreement was excellent, this rapid, commercially available qPCR panel offers benefits beyond ZCF with detection of markers for Giardia assemblages with zoonotic potential and hookworm (A. caninum) BZ resistance.
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