Evidence map›Paper›PMID 37586415›Full record

ArticleClinical and experimental immunology2024

Surface protein and functional analyses identify CD4+CD39+ TCR αβ+ and activated TCR Vδ1+ cells with distinct pro-inflammatory functions in Crohn's disease lesions.

Jan Devan, Vladimir Nosi, Julian Spagnuolo, Andrew Chancellor, Aisha Beshirova, Jose Pedro Loureiro, Alessandro Vacchini, Jan Hendrik Niess, Raffaele Calogero, Lucia Mori and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical and experimental immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Jan DevanExperimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Vladimir NosiExperimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Julian SpagnuoloExperimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Andrew ChancellorExperimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Aisha BeshirovaExperimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Jose Pedro LoureiroExperimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Alessandro VacchiniExperimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Jan Hendrik NiessGastroenterology, Department of Biomedicine, University of Basel, Clarunis, Basel, Switzerland.
Raffaele CalogeroDepartment of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy.ORCID 0000-0002-2848-628X
Lucia MoriExperimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Gennaro De LiberoExperimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Petr HruzExperimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.ORCID 0000-0003-2767-0445
University of Basel · CHUniversity of Turin · IT

Funding

Swiss National Foundation 310030-173240Uniscientia foundation and an Investigator Initiated Research IISR-2016-101920
6 · The paper itself

Abstract

Crohn's disease (CD) is a chronic immune-mediated disorder of the gastrointestinal tract. Extensive screening studies have revealed the accumulation of immune cell subsets with unique plasticity and immunoregulatory properties in patients with CD. We performed phenotypic and functional studies on inflamed and non-inflamed bioptic tissue to investigate the presence of distinct T cells in the intestinal mucosa of CD patients. We analysed hundreds of surface molecules expressed on cells isolated from the intestinal tissue of CD patients using anti-CD45 mAbs-based barcoding. A gene ontology enrichment analysis showed that proteins that regulate the activation of T cells were the most enriched group. We, therefore, designed T-cell focused multicolour flow-cytometry panels and performed clustering analysis which revealed an accumulation of activated TEM CD4+CD39+ T cells producing IL-17 and IL-21 and increased frequency of terminally differentiated TCR Vδ1+ cells producing TNF-α and IFN-γ in inflamed tissue of CD patients. The different functional capacities of CD4+ and TCR Vδ1+ cells in CD lesions indicate their non-overlapping contribution to inflammation. The abnormally high number of terminally differentiated TCR Vδ1+ cells suggests that they are continuously activated in inflamed tissue, making them a potential target for novel therapies.

Indexed as

Crohn DiseaseHumansInflammationMembrane ProteinsReceptors, Antigen, T-Cell, alpha-betaT-LymphocytesMembrane ProteinsReceptors, Antigen, T-Cell, alpha-betaCD4Crohn’s diseasegamma delta T cells

Identifiers

PMID37586415
PMCPMC10776239
OpenAlexW4385852822

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.