Evidence map›Paper›PMID 37584524›Full record

ArticleMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada2023

Rehydration of Freeze Substituted Brain Tissue for Pre-embedding Immunoelectron Microscopy.

Janeth Pérez-Garza, Emily Parrish-Mulliken, Zachary Deane, Linnaea E Ostroff

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Janeth Pérez-GarzaDepartment of Physiology and Neurobiology, University of Connecticut, 75 North Eagleville Rd. Unit 3156, Storrs, CT 06269-3156, USA.ORCID 0000-0002-3023-7741
Emily Parrish-MullikenDepartment of Physiology and Neurobiology, University of Connecticut, 75 North Eagleville Rd. Unit 3156, Storrs, CT 06269-3156, USA.
Zachary DeaneDepartment of Physiology and Neurobiology, University of Connecticut, 75 North Eagleville Rd. Unit 3156, Storrs, CT 06269-3156, USA.
Linnaea E OstroffDepartment of Physiology and Neurobiology, University of Connecticut, 75 North Eagleville Rd. Unit 3156, Storrs, CT 06269-3156, USA.ORCID 0000-0002-3348-342X

Funding

A versatile approach for highly multiplexed, high-resolution imaging of endogenous moleculesRF1MH130472 · NIMH · UNIVERSITY OF CONNECTICUT STORRS · PI OSTROFF, LINNAEA E · 2022 to 2022
$2.2M
New strategies for molecular cell-type labeling in volume electron microscopyRF1MH129269 · NIMH · UNIVERSITY OF CONNECTICUT STORRS · PI OSTROFF, LINNAEA E · 2022 to 2022
$1.1M
NIMH NIH HHS RF1 MH129269NIMH NIH HHS RF1 MH130472
6 · The paper itself

Abstract

Electron microscopy (EM) volume reconstruction is a powerful tool for investigating the fundamental structure of brain circuits, but the full potential of this technique is limited by the difficulty of integrating molecular information. High quality ultrastructural preservation is necessary for EM reconstruction, and intact, highly contrasted cell membranes are essential for following small neuronal processes through serial sections. Unfortunately, the antibody labeling methods used to identify most endogenous molecules result in compromised morphology, especially of membranes. Cryofixation can produce superior morphological preservation and has the additional advantage of allowing indefinite storage of valuable samples. We have developed a method based on cryofixation that allows sensitive immunolabeling of endogenous molecules, preserves excellent ultrastructure, and is compatible with high-contrast staining for serial EM reconstruction.

Indexed as

BrainCryopreservationFluid TherapyFreeze SubstitutionFreezingMicroscopy, Immunoelectronantibody labelingelectron microscopyEM volume reconstructionfreeze substitutionhigh-pressure freezingneuroanatomy

Identifiers

PMID37584524
PMCPMC10541149

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.