Evidence map›Paper›PMID 37584027›Full record

ArticleAPL bioengineering2023

User-friendly microfluidic system reveals native-like morphological and transcriptomic phenotypes induced by shear stress in proximal tubule epithelium.

Natalie N Khalil, Andrew P Petersen, Cheng J Song, Yibu Chen, Kaelyn Takamoto, Austin C Kellogg, Elaine Zhelan Chen, Andrew P McMahon, Megan L McCain

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in APL bioengineering, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Natalie N KhalilAlfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, California 90089, USA.ORCID https://orcid.org/0000-0003-1209-2718
Andrew P PetersenAlfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, California 90089, USA.ORCID https://orcid.org/0000-0003-0765-5248
Cheng J SongORCID https://orcid.org/0000-0001-9541-8909
Yibu ChenUSC Libraries Bioinformatics Service, University of Southern California, Los Angeles, California 90089, USA.ORCID https://orcid.org/0000-0002-7889-6850
Kaelyn TakamotoAlfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, California 90089, USA.ORCID https://orcid.org/0009-0002-2984-1172
Austin C KelloggAlfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, California 90089, USA.ORCID https://orcid.org/0009-0001-2216-1206
Elaine Zhelan ChenAlfred E. Mann Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, California 90089, USA.ORCID https://orcid.org/0000-0003-4061-7921
Andrew P McMahonORCID https://orcid.org/0000-0002-3779-1729
Megan L McCainORCID https://orcid.org/0000-0003-1908-6783
University of Southern California · US

Funding

Establishing Mechanisms of Human Proximal Tubule Regeneration in an Engineered Organ on Chip PlatformR21EB025534 · NIBIB · UNIVERSITY OF SOUTHERN CALIFORNIA · PI MCCAIN, MEGAN LAURA, MCMAHON, ANDREW P. · 2017 to 2018
$427k
NIBIB NIH HHS R21 EB025534
6 · The paper itself

Abstract

Drug-induced nephrotoxicity is a leading cause of drug attrition, partly due to the limited relevance of pre-clinical models of the proximal tubule. Culturing proximal tubule epithelial cells (PTECs) under fluid flow to mimic physiological shear stress has been shown to improve select phenotypes, but existing flow systems are expensive and difficult to implement by non-experts in microfluidics. Here, we designed and fabricated an accessible and modular flow system for culturing PTECs under physiological shear stress, which induced native-like cuboidal morphology, downregulated pathways associated with hypoxia, stress, and injury, and upregulated xenobiotic metabolism pathways. We also compared the expression profiles of shear-dependent genes in our

Identifiers

PMID37584027
PMCPMC10424157
OpenAlexW4385630512

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.