ArticleBMC infectious diseases2023
Genotype diversity of brucellosis agents isolated from humans and animals in Greece based on whole-genome sequencing.
Article in BMC infectious diseases, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 2 of them syntheses that pooled it.
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14 citing papers in PubMed, 2 syntheses or guidelines pooled it, 18 citations in OpenAlex.
- A Comprehensive Meta-Analysis on Antimicrobial Resistance Patterns of the Two MajorTransboundary and emerging diseases · 2025Pooled it
- Unravelling the challenge of cotrimoxazole and rifampin resistance in B. melitensis and B. abortus: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2024Pooled it
- Host and Pathogen Genetic Determinants of Brucellosis in Veterinary-One Health Interface: A Review.Veterinary sciences · 2026Review
- Study of epidemiological and molecular characteristics of Brucella strains circulating in Kazakhstan.Veterinary research communications · 2025Article
- Genomic analysis of Brucella melitensis isolates recovered from humans in south Tunisia over 35 years between 1988 and 2022.BMC microbiology · 2025Article
- The history of brucellosis in the Middle East: insights for contemporary health challenges.Frontiers in microbiology · 2025Review
- Limited genomic diversity and convergent adaptation ofFrontiers in microbiology · 2025Article
- Whole-Genome Sequencing of Brucella melitensis Isolates from Kuwait for the Identification of Biovars, Variants, and Relationship within a Biovar.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2025Article
- The European Union One Health 2023 Zoonoses report.EFSA journal. European Food Safety Authority · 2024Article
- Whole-genome sequencing-based analysis of Brucella species isolated from ruminants in various regions of Türki̇ye.BMC infectious diseases · 2024Article
- Genomic analysis ofEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- Epidemiological Investigation of Animal Brucellosis in Domestic Ruminants in Greece from 2015 to 2022 and Genetic Characterization of Prevalent Strains.Pathogens (Basel, Switzerland) · 2024Article
- Combination ofFrontiers in microbiology · 2024Article
- Detection of Virulence-Associated Genes amongPathogens (Basel, Switzerland) · 2023Article
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14 authors at 6 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundBrucellosis is a zoonotic disease whose causative agent, Brucella spp., is endemic in many countries of the Mediterranean basin, including Greece. Although the occurrence of brucellosis must be reported to the authorities, it is believed that the disease is under-reported in Greece, and knowledge about the genomic diversity of brucellae is lacking.
methodsThus, 44 Brucella isolates, primarily B. melitensis, collected between 1999 and 2009 from humans and small ruminants in Greece were subjected to whole genome sequencing using short-read technology. The raw reads and assembled genomes were used for in silico genotyping based on single nucleotide substitutions and alleles. Further, specific genomic regions encoding putative virulence genes were screened for characteristic nucleotide changes, which arose in different genotype lineages.
resultsIn silico genotyping revealed that the isolates belonged to three of the known sublineages of the East Mediterranean genotype. In addition, a novel subgenotype was identified that was basal to the other East Mediterranean sublineages, comprising two Greek strains. The majority of the isolates can be assumed to be of endemic origin, as they were clustered with strains from the Western Balkans or Turkey, whereas one strain of human origin could be associated with travel to another endemic region, e.g. Portugal. Further, nucleotide substitutions in the housekeeping gene rpoB and virulence-associated genes were detected, which were characteristic of the different subgenotypes. One of the isolates originating from an aborted bovine foetus was identified as B. abortus vaccine strain RB51.
conclusionThe results demonstrate the existence of several distinct persistent Brucella sp. foci in Greece. To detect these and for tracing infection chains, extensive sampling initiatives are required.
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