Evidence map›Paper›PMID 37580399›Full record

ArticleScientific reports2023

Diffusible signal factors (DSFs) bind and repress VirF, the leading virulence activator of Shigella flexneri.

Rita Trirocco, Martina Pasqua, Angela Tramonti, Bianca Colonna, Alessandro Paiardini, Gianni Prosseda

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.4field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
  3. Gut microbes · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Rita TriroccoInstitute Pasteur Italia, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome, p.le Aldo Moro 5, 00185, Rome, Italy.
Martina PasquaInstitute Pasteur Italia, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome, p.le Aldo Moro 5, 00185, Rome, Italy.
Angela TramontiInstitute of Molecular Biology and Pathology, National Research Council, Rome, Italy.
Bianca ColonnaInstitute Pasteur Italia, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome, p.le Aldo Moro 5, 00185, Rome, Italy.
Alessandro PaiardiniDepartment of Biochemical Sciences, Sapienza University of Rome, p.le Aldo Moro 5, 00185, Rome, Italy.
Gianni ProssedaInstitute Pasteur Italia, Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome, p.le Aldo Moro 5, 00185, Rome, Italy. gianni.prosseda@uniroma1.it.
Istituto Pasteur · ITSapienza University of Rome · ITNational Research Council · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Shigella, the aetiological agent of human bacillary dysentery, controls the expression of its virulence determinants through an environmentally stimulated cascade of transcriptional activators. VirF is the leading activator and is essential for proper virulence expression. In this work, we report on in vitro and in vivo experiments showing that two autoinducers of the DSF family, XcDSF and BDSF interact with the jelly roll module of VirF causing its inhibition and affecting the expression of the entire virulence system of Shigella, including its ability to invade epithelial cells. We propose a molecular model explaining how the binding of XcDSF and BDSF causes inhibition of VirF by preventing its dimerization. Overall, our experimental results suggest that XcDSF and BDSF may contribute to "colonisation resistance" in the human gut or, alternatively, may be exploited for the fine-tuning of Shigella virulence expression as the bacterium migrates from the lumen to approach the intestinal mucosa. Our findings also stress how a detailed understanding of the interaction of DSF ligands with VirF may contribute to the rational development of innovative antivirulence drugs to treat shigellosis.

Indexed as

Dysentery, BacillaryShigellaBacterial ProteinsGene Expression Regulation, BacterialHumansInterferon Regulatory FactorsShigella flexneriViral ProteinsVirulenceVirulence FactorsBacterial ProteinsInterferon Regulatory Factorsviral interferon regulatory factorsViral ProteinsVirulence Factors

Identifiers

PMID37580399
PMCPMC10425336
OpenAlexW4385809900

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.