ReviewACS environmental Au2021
Wastewater-Based Epidemiology for Community Monitoring of SARS-CoV-2: Progress and Challenges.
Review in ACS environmental Au, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 67 citations in OpenAlex.
- Simultaneous detection of multiple viral pathogens in vacuumed dust from different building types.Building and environment · 2026Article
- Remotely Controlled Continuous Surveillance of Viral RNA in Wastewater Using a LoRa Network.IEEE internet of things journal · 2026Article
- Detection of the BK virus in urban wastewater inlets into the Karun River.Iranian journal of microbiology · 2025Article
- Machine learning comparison for biomarker level estimation in wastewater dynamics monitoring.Scientific reports · 2025Article
- Wastewater Speaks: Evaluating SARS-CoV-2 Surveillance, Sampling Methods, and Seasonal Infection Trends on a University Campus.Microorganisms · 2025Article
- Hydrological and physicochemical parameters associated with SARS-CoV-2 and pepper mild mottle virus wastewater concentrations for a large-combined sewer system.Journal of water and health · 2025Article
- Integrated PRRSV prevention and control strategy based on the One Health concept: across the boundaries of virology, ecology and public health.Frontiers in microbiology · 2025Review
- Environmental Surveillance of Flood Control Infrastructure Impacted by Unsheltered Individuals Leads to the Detection of SARS-CoV-2 and Novel Mutations in the Spike Gene.Environmental science & technology letters · 2024Article
- Real-Time On-Site Monitoring of Viruses in Wastewater Using NanotrapBiosensors · 2024Article
- Quantification and Differentiation of SARS-CoV-2 Variants in Wastewater for Surveillance.Environment & health (Washington, D.C.) · 2023Article
- Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle.Trends in analytical chemistry : TRAC · 2023Review
- An efficient method to enhance recovery and detection of SARS-CoV-2 RNA in wastewater.Journal of environmental sciences (China) · 2023Article
- RNA extraction-free workflow integrated with a single-tube CRISPR-Cas-based colorimetric assay for rapid SARS-CoV-2 detection in different environmental matrices.Journal of hazardous materials · 2023Article
- An improved method for determining frequency of multiple variants of SARS-CoV-2 in wastewater using qPCR assays.The Science of the total environment · 2023Article
- SARS-CoV-2 in the environment: Contamination routes, detection methods, persistence and removal in wastewater treatment plants.The Science of the total environment · 2023Review
- Sensitivity of wastewater surveillance: What is the minimum COVID-19 cases required in population for SARS-CoV-2 RNA to be detected in wastewater?Journal of environmental sciences (China) · 2023Article
- Biomarker selection strategies based on compound stability in wastewater-based epidemiology.Environmental science and pollution research international · 2023Review
- Spectrum of environmental surveillance of SARS-CoV-2 fragments: Questions, quests, and conquest.Current opinion in environmental science & health · 2022Review
- Comparison of RT-dPCR and RT-qPCR and the effects of freeze-thaw cycle and glycine release buffer for wastewater SARS-CoV-2 analysis.Scientific reports · 2022Article
- An opinion on Wastewater-Based Epidemiological Monitoring (WBEM) with Clinical Diagnostic Test (CDT) for detecting high-prevalence areas of community COVID-19 Infections.Current opinion in environmental science & health · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wastewater-based epidemiology (WBE) is useful for the surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in communities, complementing clinical diagnostic testing of individuals. In this Review, we summarize recent progress and highlight remaining challenges in monitoring SARS-CoV-2 RNA in wastewater systems for community and environmental surveillance. Very low concentrations of viral particles and RNA present in the complicated wastewater and sewage sample matrix require efficient sample processing and sensitive detection. We discuss advantages and limitations of available methods for wastewater sample processing, including collection, separation, enrichment, RNA extraction, and purification. Efficient extraction of the viral RNA and removal of interfering sample matrices are critical to the subsequent reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for sensitive detection of SARS-CoV-2 in wastewater. We emphasize the importance of implementing appropriate controls and method validation, which include the use of surrogate viruses for assessing extraction efficiency and normalization against measurable chemical and biological components in wastewater. Critical analysis of the published studies reveals imperative research needs for the development, validation, and standardization of robust and sensitive methods for quantitative detection of viral RNA and proteins in wastewater for WBE.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.