Evidence map›Paper›PMID 37578717›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Measuring NLR Oligomerization III: Detection of NLRP3 and NLRC4 Complex by Bioluminescence Resonance Energy Transfer.

Fátima Martín-Sánchez, Alejandro Peñín-Franch, Diego Angosto-Bazarra, Ana Tapia-Abellán, Vincent Compan, Pablo Pelegrín

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Fátima Martín-SánchezInflammation and Experimental Surgery Unit, Institute for Bio-Health Research of Murcia, Clinical University Hospital Virgen de la Arrixaca, Murcia, Spain.
Alejandro Peñín-FranchBiomedical Research Institute of Murcia, Murcia, Spain.
Diego Angosto-BazarraBiomedical Research Institute of Murcia, Murcia, Spain.
Ana Tapia-AbellánBiomedical Research Institute of Murcia, Murcia, Spain.
Vincent CompanInstitut de Génomique Fonctionnelle, Labex ICST, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Université Montpellier, Montpellier, France.
Pablo PelegrínBiomedical Research Institute of Murcia, Murcia, Spain. pablopel@um.es.
Instituto Murciano de Investigación Biosanitaria · ESUniversidad de Murcia · ESUniversité de Montpellier · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bioluminescent resonance energy transfer (BRET) is a natural phenomenon resulting from a non-radiative energy transfer between a bioluminescent donor (Renilla luciferase) and a fluorescent protein acceptor. BRET signal is dependent on the distance and the orientation between the donor and the acceptor and could be used to study protein-protein interactions and conformational changes within proteins at real-time in living cells. This protocol describes the use of BRET technique to study NLRP3 oligomerization in living cells before and during NLRP3 inflammasome activation.

Indexed as

NLR Family, Pyrin Domain-Containing 3 ProteinProteinsBioluminescence Resonance Energy Transfer TechniquesEnergy TransferLuciferases, RenillaLuminescent MeasurementsLuciferases, RenillaNLR Family, Pyrin Domain-Containing 3 ProteinProteinsBioluminescenceBRETNLRC4NLRP3Sensor

Identifiers

PMID37578717
OpenAlexW4385798146

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.