Evidence map›Paper›PMID 37577723›Full record

ArticlebioRxiv : the preprint server for biology2023

Defining Mitochondrial Cristae Morphology Changes Induced by Aging in Brown Adipose Tissue.

Amber Crabtree, Kit Neikirk, Andrea G Marshall, Larry Vang, Aaron J Whiteside, Qiana Williams, Christopher T Altamura, Trinity Celeste Owens, Dominique Stephens, Bryanna Shao and 19 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

29 authors at 9 institutions in 2 countries.

Amber CrabtreeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Kit NeikirkDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Andrea G MarshallDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Larry VangDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Aaron J WhitesideDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Qiana WilliamsDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Christopher T AltamuraDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Trinity Celeste OwensDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Dominique StephensDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Bryanna ShaoDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Alice KohDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Mason KillionDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Edgar Garza LopezDepartment of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA.
Jacob LamDepartment of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA.
Ben RodriguezDepartment of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA.
Margaret MungaiDepartment of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA.
Jade StanleyDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
E Danielle DeanDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Ho-Jin KohDepartment of Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
Jennifer A GaddyDepartment of Biological Sciences, Tennessee State University, Nashville, TN 37209.
Estevão ScudeseLaboratory of Biosciences of Human Motricity (LABIMH) of the Federal University of State of Rio de Janeiro (UNIRIO), Rio de Janeiro, Brazil; Sport Sciences and Exercise Laboratory (LaCEE), Catholic University of Petrópolis (UCP), Brazil.
Mariya SweetwyneDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, 98195, USA.
Jamaine DavisDepartment of Biochemistry, Cancer Biology, Neuroscience, Pharmacology, Meharry Medical College, Nashville, TN 37208 USA.
Elma ZaganjorDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Sandra A MurrayDepartment of Cell Biology, University of Pittsburgh; Pittsburg h, PA, 15261 USA.
Prasanna KattiNational Heart, Lung and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA.
Steven M DamoDepartment of Life and Physical Sciences, Fisk University, Nashville, TN, 37208, USA.
Zer VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Antentor HintonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Vanderbilt University · USUniversity of Iowa · USMeharry Medical College · USNational Institutes of Health · USUnited States Department of Veterans Affairs · USUniversidade Católica de Petrópolis · BRUniversity of Pittsburgh · USUniversity of Washington · USVanderbilt University Medical Center · US

Funding

Vanderbilt Diabetes Research CenterP30DK020593 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI OWEN P MCGUINNESS · 2012 to 2026
$29.3M
MULTIDISCIPLINARY TRAINING IN MOLECULAR ENDOCRINOLOGYT32DK007563 · NIDDK · VANDERBILT UNIVERSITY · PI Richard M O'Brien · 1988 to 2026
$15.9M
PRIDE: Functional and Translational Genomics of Blood DisordersR25HL106365 · NHLBI · AUGUSTA UNIVERSITY · PI PACE, BETTY SUE · 2010 to 2024
$5.2M
Integrated Training in Engineering and DiabetesT32DK101003 · NIDDK · VANDERBILT UNIVERSITY · PI Jamey D. Young · 2014 to 2026
$3.9M
Arginine regulation of alpha cell proliferation and functionR01DK132669 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Erika Danielle Dean · 2022 to 2026
$2.2M
The Role of Arginine Transport on Pancreatic Alpha Cell Proliferation and FunctionF31DK134158 · NIDDK · VANDERBILT UNIVERSITY · PI STANLEY, JADE ELISE · 2023 to 2024
$67k
NHLBI NIH HHS R25 HL106365NIDDK NIH HHS F31 DK134158NIDDK NIH HHS P30 DK020593NIDDK NIH HHS R01 DK132669NIDDK NIH HHS T32 DK007563NIDDK NIH HHS T32 DK101003
6 · The paper itself

Abstract

Mitochondria are required for energy production and even give brown adipose tissue (BAT) its characteristic color due to their high iron content and abundance. The physiological function and bioenergetic capacity of mitochondria are connected to the structure, folding, and organization of its inner-membrane cristae. During the aging process, mitochondrial dysfunction is observed, and the regulatory balance of mitochondrial dynamics is often disrupted, leading to increased mitochondrial fragmentation in aging cells. Therefore, we hypothesized that significant morphological changes in BAT mitochondria and cristae would be present with aging. We developed a quantitative three-dimensional (3D) electron microscopy approach to map cristae network organization in mouse BAT to test this hypothesis. Using this methodology, we investigated the 3D morphology of mitochondrial cristae in adult (3-month) and aged (2-year) murine BAT tissue via serial block face-scanning electron microscopy (SBF-SEM) and 3D reconstruction software for manual segmentation, analysis, and quantification. Upon investigation, we found increases in mitochondrial volume, surface area, and complexity and decreased sphericity in aged BAT, alongside significant decreases in cristae volume, area, perimeter, and score. Overall, these data define the nature of the mitochondrial structure in murine BAT across aging. Abstract Figure:

Identifiers

PMID37577723
PMCPMC10418056
OpenAlexW4376618801

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.