Evidence map›Paper›PMID 37577521›Full record

ArticlebioRxiv : the preprint server for biology2023

Flavinated SDHA Underlies the Change in Intrinsic Optical Properties of Oral Cancers.

Tomoko Marumo, Chima V Maduka, Evran Ural, Ehsanul Hoque Apu, Seock-Jin Chung, Nynke S van den Berg, Quan Zhou, Brock A Martin, Eben L Rosenthal, Takahiko Shibahara and 1 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Tomoko MarumoDepartment of Oral and Maxillofacial Surgery, Tokyo Dental College, 2-9-18 Kanda-Misakicho, Chiyoda-ku, Tokyo 101-0061, Japan.
Chima V MadukaDepartment of Biomedical Engineering, Michigan State University, East Lansing, MI 48824, USA.
Evran UralDepartment of Biomedical Engineering, Michigan State University, East Lansing, MI 48824, USA.
Ehsanul Hoque ApuDepartment of Biomedical Engineering, Michigan State University, East Lansing, MI 48824, USA.
Seock-Jin ChungDepartment of Biomedical Engineering, Michigan State University, East Lansing, MI 48824, USA.
Nynke S van den BergDepartment of Otolaryngology - Division of Head and Neck Surgery, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA.
Quan ZhouDepartment of Otolaryngology - Division of Head and Neck Surgery, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA.
Brock A MartinDepartment of Pathology, Stanford University School of Medicine, 3100 Pasteur Drive, Stanford, CA 94305, USA.
Eben L RosenthalDepartment of Otolaryngology - Division of Head and Neck Surgery, Stanford University School of Medicine, 269 Campus Drive, Stanford, CA 94305, USA.
Takahiko ShibaharaDepartment of Oral and Maxillofacial Surgery, Tokyo Dental College, 2-9-18 Kanda-Misakicho, Chiyoda-ku, Tokyo 101-0061, Japan.
Christopher H ContagDepartment of Biomedical Engineering, Michigan State University, East Lansing, MI 48824, USA.
Michigan State University · USStanford Medicine · USTokyo Dental College · JPMichigan Medicine · USStanford University · US

Funding

Raman Molecular Imaging for Early Detection of Colon CancerR01CA182043 · NCI · STANFORD UNIVERSITY · PI CONTAG, CHRISTOPHER H · 2015 to 2020
$2.8M
NCI NIH HHS R01 CA182043
6 · The paper itself

Abstract

The molecular basis of reduced autofluorescence in oral squamous cell carcinoma (OSCC) cells relative to normal cells has been speculated to be due to lower levels of free flavin adenine dinucleotide (FAD). This speculation, along with differences in the intrinsic optical properties of extracellular collagen, lie at the foundation of the design of currently-used clinical optical detection devices. Here, we report that free FAD levels may not account for differences in autofluorescence of OSCC cells, but that the differences relate to FAD as a co-factor for flavination. Autofluorescence from a 70 kDa flavoprotein, succinate dehydrogenase A (SDHA), was found to be responsible for changes in optical properties within the FAD spectral region with lower levels of flavinated SDHA in OSCC cells. Since flavinated SDHA is required for functional complexation with succinate dehydrogenase B (SDHB), decreased SDHB levels were observed in human OSCC tissue relative to normal tissues. Accordingly, the metabolism of OSCC cells was found to be significantly altered relative to normal cells, revealing vulnerabilities for both diagnosis and targeted therapy. Optimizing non-invasive tools based on optical and metabolic signatures of cancers will enable more precise and early diagnosis leading to improved outcomes in patients.

Indexed as

Autofluorescenceflavin adenine dinucleotide (FAD)metabolismoral squamous cell carcinoma (OSCC)succinate dehydrogenase

Identifiers

PMID37577521
PMCPMC10418065
OpenAlexW4385409926

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.