Evidence map›Paper›PMID 37577166›Full record

ArticleMethodsX2023

Extracting accurate infrared lineshapes from weak vibrational probes at low concentrations.

Raiza N A Maia, Sunayana Mitra, Carlos R Baiz

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MethodsX, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Raiza N A MaiaDepartment of Chemistry, University of Texas at Austin, Austin, TX 78712-1224, USA.
Sunayana MitraDepartment of Chemistry, University of Texas at Austin, Austin, TX 78712-1224, USA.
Carlos R BaizDepartment of Chemistry, University of Texas at Austin, Austin, TX 78712-1224, USA.
The University of Texas at Austin · US

Funding

Protein structure and dynamics in ultra-heterogeneous environments-RenewalR35GM133359 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Carlos Raul Baiz · 2019 to 2026
$1.8M
NIGMS NIH HHS R35 GM133359
6 · The paper itself

Abstract

Fourier-transform infrared (FTIR) spectroscopy using vibrational probes is an ideal tool to detect changes in structure and local environments within biological molecules. However, challenges arise when dealing with weak infrared probes, such as thiocyanates, due to their inherent low signal strengths and overlap with solvent bands. In this protocol we demonstrate:•A streamlined approach for the precise extraction of weak infrared absorption lineshapes from a strong solvent background.•A protocol combining a spectral filter, background modeling, and subtraction.•Our methodology successfully extracts the CN stretching mode peak from methyl thiocyanate at remarkably low concentrations (0.25 mM) in water, previously a challenge for FTIR spectroscopy.This approach offers valuable insights and tools for more accurate FTIR measurements using weak vibrational probes. This enhanced precision can potentially enable new approaches to enhance our understanding of protein structure and dynamics in solution.

Indexed as

Analysis of weak vibrational probesData processingFTIRProtein structureSpectroscopyThiocyanateVibrational modes

Identifiers

PMID37577166
PMCPMC10416016
OpenAlexW4385411684

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.