ArticleEcology and evolution2023
DNA isolation and genome sequence of the 134-year-old holotype specimen of
Article in Ecology and evolution, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed, 3 citations in OpenAlex.
- Short-read genome skimming enables molecular barcoding of old myxomycete collections.IMA fungus · 2026Article
- The First Whole Genome Sequencing of Historical Lichen Specimens Enables Genome-Wide Analysis of Fungal and Algal Symbionts.Ecology and evolution · 2025Article
- DNA isolation and genome sequence of the 134-year-old holotype specimen ofEcology and evolution · 2023Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Molecular characterization of type specimens is a powerful tool used in clarifying species identity/circumscription, as well as establishing the taxonomic and phylogenetic status of organisms in question. However, DNA sequencing of aged herbarium collections can be a challenge due to the quantity and quality of DNA still present in the specimens. Herein, we report a custom DNA isolation protocol suitable for processing minute quantities of old specimen tissue and its utilization via high-throughput sequencing technologies to obtain, for the first time, the genome assembly of the 134-year-old holotype of
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Registered trials
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