Evidence map›Paper›PMID 37573325›Full record

ArticleJournal of translational medicine2023

Decreased cyclooxygenase-2 associated with impaired megakaryopoiesis and thrombopoiesis in primary immune thrombocytopenia.

Xibing Zhuang, Pengcheng Xu, Yang Ou, Xia Shao, Ying Li, Yanna Ma, Shanshan Qin, Fanli Hua, Yanxia Zhan, Lili Ji and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of translational medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.5field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Xibing Zhuang *Department of Hematology, Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai 180 Fenglin Rd, Shanghai, 200032, China.
Pengcheng Xu *Center for Tumor Diagnosis & Therapy, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
Yang OuDepartment of Hematology, Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai 180 Fenglin Rd, Shanghai, 200032, China.
Xia ShaoDepartment of Hematology, Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai 180 Fenglin Rd, Shanghai, 200032, China.
Ying LiCenter for Tumor Diagnosis & Therapy, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
Yanna MaCenter for Tumor Diagnosis & Therapy, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
Shanshan QinDepartment of Hematology, Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai 180 Fenglin Rd, Shanghai, 200032, China.
Fanli HuaDepartment of Hematology, Zhongshan Hospital Qingpu Branch, Fudan University, Shanghai, 201700, China.
Yanxia ZhanDepartment of Hematology, Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai 180 Fenglin Rd, Shanghai, 200032, China.
Lili JiDepartment of Hematology, Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai 180 Fenglin Rd, Shanghai, 200032, China.
Tiankui QiaoCenter for Tumor Diagnosis & Therapy, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
Hao ChenDepartment of Thoracic Surgery, Zhongshan Hospital Xuhui Branch, Fudan University, Shanghai, 200031, China.
Yunfeng ChengDepartment of Hematology, Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai 180 Fenglin Rd, Shanghai, 200032, China. yfcheng@fudan.edu.cn.ORCID http://orcid.org/0000-0002-9581-1134
Sun Yat-sen University · CNJinshan Hospital of Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCyclooxygenase (COX)-2 is a rate-limiting enzyme in the biosynthesis of prostanoids, which is mostly inducible by inflammatory cytokines. The participation of COX-2 in the maturation of megakaryocytes has been reported but barely studied in primary immune thrombocytopenia (ITP).

methodsThe expressions of COX-2 and Caspase-1, Caspase-3 and Caspase-3 p17 subunit in platelets from ITP patients and healthy controls (HC), and the expressions of COX-2 and CD41 in bone marrow (BM) of ITP patients were measured and analyzed for correlations. The effects of COX-2 inhibitor on megakaryopoiesis and thrombopoiesis were assessed by in vitro culture of Meg01 cells and murine BM-derived megakaryocytes and in vivo experiments of passive ITP mice.

resultsThe expression of COX-2 was decreased and Caspase-1 and Caspase-3 p17 were increased in platelets from ITP patients compared to HC. In platelets from ITP patients, the COX-2 expression was positively correlated with platelet count and negatively correlated to the expression of Caspase-1. In ITP patients BM, the expression of CD41 was positively correlated with the expression of COX-2. COX-2 inhibitor inhibited the count of megakaryocytes and impaired the maturation and platelet production in Meg01 cells and bone marrow-derived megakaryocytes. COX-2 inhibitor aggravated thrombocytopenia and damaged megakaryopoiesis in ITP murine model.

conclusionCOX-2 plays a vital role in the physiologic and pathologic conditions of ITP by intervening the survival of platelets and impairing the megakaryopoiesis and thrombopoiesis of megakaryocytes.

Indexed as

Purpura, Thrombocytopenic, IdiopathicThrombopoiesisAnimalsBlood PlateletsCaspase 3Cyclooxygenase 2Cyclooxygenase 2 InhibitorsMegakaryocytesMiceCaspase 3Cyclooxygenase 2Cyclooxygenase 2 InhibitorsPtgs2 protein, mouseCyclooxygenase-2MegakaryopoiesisPrimary immune thrombocytopeniaThrombopoiesis

Identifiers

PMID37573325
PMCPMC10423426
OpenAlexW4385783881

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.