Evidence map›Paper›PMID 37573307›Full record

ArticleBMC microbiology2023

Boxwood phyllosphere fungal and bacterial communities and their differential responses to film-forming anti-desiccants.

Xiaoping Li, Olanike Omolehin, Ginger Hemmings, Hsien Tzer Tseng, Amanda Taylor, Chad Taylor, Ping Kong, Margery Daughtrey, Douglas Luster, Fred Gouker and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xiaoping LiHampton Roads Agricultural Research and Extension Center, Virginia Tech, Virginia Beach, VA, USA. lixiaopi@vt.edu.
Olanike OmolehinHampton Roads Agricultural Research and Extension Center, Virginia Tech, Virginia Beach, VA, USA.
Ginger HemmingsPlant Industry Division, North Carolina Department of Agriculture and Consumer Services, Dobson, NC, USA.
Hsien Tzer TsengPlant Industry Division, North Carolina Department of Agriculture and Consumer Services, Raleigh, NC, USA.
Amanda TaylorNorth Carolina University Cooperative Extension, Morganton, NC, USA.
Chad TaylorPlant Industry Division, North Carolina Department of Agriculture and Consumer Services, Boone, NC, USA.
Ping KongHampton Roads Agricultural Research and Extension Center, Virginia Tech, Virginia Beach, VA, USA.
Margery DaughtreyLong Island Horticultural Research and Extension Center, Cornell University, Riverhead, NY, USA.
Douglas LusterForeign Disease-Weed Science Research Unit, USDA-ARS-NEA, Fort Detrick, MD, USA.
Fred GoukerU.S. National Arboretum, Floral and Nursery Plants Research Unit, USDA-ARS, Beltsville, MD, USA.
Chuanxue HongHampton Roads Agricultural Research and Extension Center, Virginia Tech, Virginia Beach, VA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAnti-desiccant is a class of agrochemicals widely used to protect plants from water stresses, rapid temperature variations, heat and sunburn, frost and freeze damages, transplant shock, and pathogen and pest attack. Although anti-desiccants are generally considered non-toxic to organisms, it is unclear whether they may impact the phyllosphere microbial communities. In this study, three film-forming anti-desiccant products, TransFilm, Vapor Gard, and Wilt-Pruf were applied to the canopy of two boxwood cultivars 'Vardar Valley' and 'Justin Brouwers' on April 13 and August 26, 2021. Shoot samples were collected from boxwood plants treated with each of the three products, as well as nontreated control on June 16, August 26 (before the second treatment), and October 18. Microbial and plant genomic DNA was isolated together and 16S rRNA gene and the extended internal transcribed spacer regions were amplified with PCR and sequenced on a Nanopore MinION platform for bacterial and fungal identification.

resultsBacterial communities were more diverse than fungal communities. At the phylum level, the boxwood phyllosphere was dominated by Proteobacteria and Ascomycota; at the genus level, Methylobacterium and Shiraia were the most abundant bacteria and fungi, respectively. Among the three film-forming anti-desiccants, Vapor Gard and Wilt-Pruf had more impact than TransFilm on the microbial communities. Specifically, broader impacts were observed on fungal than bacterial community composition and structure, with most affected fungi being suppressed while bacteria promoted.

conclusionThis study addressed several major knowledge gaps regarding boxwood phyllosphere microbiota and the impact of anti-desiccants on plant microbiome. We identified diverse microbial communities of boxwood, a major evergreen woody crop and an iconic landscape plant. We also found differential effects of three film-forming anti-desiccants on the composition and structure of bacterial and fungal communities. These findings advanced our understanding of the associated microbiome of this landmark plant, enabling growers to fully utilize the potentials of microbiome and three anti-desiccants in improving boxwood health and productivity.

Indexed as

AscomycotaBuxusMicrobiotaBacteriaPlantsRNA, Ribosomal, 16SRNA, Ribosomal, 16SAnti-transpirantBoxwoodCrop protectionDi-1-p-mentheneNanopore MinION sequencingPhytobiomesSystems approach

Identifiers

PMID37573307
PMCPMC10422719

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.