ArticleMed (New York, N.Y.)2023
Organoid co-culture model of the human endometrium in a fully synthetic extracellular matrix enables the study of epithelial-stromal crosstalk.
Article in Med (New York, N.Y.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 90 citations in OpenAlex.
- MASLD, diabetes and PMOS across the female life stages.Diabetologia · 2026Review
- Organoids: Current Applications and Future Directions.MedComm · 2026Review
- Extracellular matrix composition and stiffness regulates progesterone responsiveness and decidualization.iScience · 2026Article
- Engineering the human endometrium at the intersection of development and reproduction.Nature biomedical engineering · 2026Review
- Bioprinted Human Umbilical Cord Mesenchymal Stem Cell-laden Microfiber-derived Extracellular Vesicles Promote Endometrial Repair and Fertility Recovery in Intrauterine Adhesions.Stem cell reviews and reports · 2026Article
- Modeling human embryo adhesion using a microfluidic platform.Science advances · 2026Article
- Development and characterization of functional sheep endometrial luminal epithelial organoids.Veterinary research · 2026Article
- Deconstructing cancer in 3D: models, mechanisms, and personalized solutions.Molecular cancer · 2026Review
- Exploring endometriosis through 3DMaterials today. Bio · 2026Review
- Development of a Synthetic Hydrogel to Foster Microvascularization of an Endometriosis Microphysiological System.Advanced healthcare materials · 2026Article
- Human inflammatory bowel disease-on-a-chip for modelling disease progression, cancer initiation and sex-specific effects.Nature biomedical engineering · 2026Article
- Article
- Somatic mutations in endometrial epithelium: biological insights and emerging in vitro models.Molecular human reproduction · 2026Review
- Three-dimensional patient-derived endometriosis model for drug evaluation.Acta obstetricia et gynecologica Scandinavica · 2026Article
- The Intersecting Physical Mechanisms That Regulate Cell Viability in 3D Synthetic Hydrogels.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Equine endometrial organoids preserve tissue structure and cycle-stage transcriptional identity†.Biology of reproduction · 2026Article
- Engineering the Human Endometrial-Embryo Interface: Breakthroughs in 3D Uterine Models.Biomolecules · 2026Review
- Highly Tunable and Cell-Remodelable Thiol-ene Alginate-Peptide Crosslinked Hydrogels to Recreate Cellular and Organoid Microenvironments for Biofabrication.Advanced healthcare materials · 2026Article
- Organoids for Metabolic Disease Modeling.Journal of inherited metabolic disease · 2026Review
- Female Reproductive Tract Organ-on-Chips: Modeling Barrier Function and Drug Transport.Pharmaceutics · 2026Review
12 more citing papers are in PubMed but not listed here.
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10 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundThe human endometrium undergoes recurring cycles of growth, differentiation, and breakdown in response to sex hormones. Dysregulation of epithelial-stromal communication during hormone-mediated signaling may be linked to myriad gynecological disorders for which treatments remain inadequate. Here, we describe a completely defined, synthetic extracellular matrix that enables co-culture of human endometrial epithelial and stromal cells in a manner that captures healthy and disease states across a simulated menstrual cycle.
methodsWe parsed cycle-dependent endometrial integrin expression and matrix composition to define candidate cell-matrix interaction cues for inclusion in a polyethylene glycol (PEG)-based hydrogel crosslinked with matrix metalloproteinase-labile peptides. We semi-empirically screened a parameter space of biophysical and molecular features representative of the endometrium to define compositions suitable for hormone-driven expansion and differentiation of epithelial organoids, stromal cells, and co-cultures of the two cell types.
findingsEach cell type exhibited characteristic morphological and molecular responses to hormone changes when co-encapsulated in hydrogels tuned to a stiffness regime similar to the native tissue and functionalized with a collagen-derived adhesion peptide (GFOGER) and a fibronectin-derived peptide (PHSRN-K-RGD). Analysis of cell-cell crosstalk during interleukin 1B (IL1B)-induced inflammation revealed dysregulation of epithelial proliferation mediated by stromal cells.
conclusionsAltogether, we demonstrate the development of a fully synthetic matrix to sustain the dynamic changes of the endometrial microenvironment and support its applications to understand menstrual health and endometriotic diseases.
fundingThis work was supported by The John and Karine Begg Foundation, the Manton Foundation, and NIH U01 (EB029132).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.