Evidence map›Paper›PMID 37572510›Full record

ArticleDiagnostic microbiology and infectious disease2023

The limit of detection of the BioFire® FilmArray® gastrointestinal panel for the foodborne parasite Cyclospora cayetanensis.

Anna Peterson, Travis Richins, Katelyn Houghton, Margarita Mishina, Suresh Sharma, Suryaprakash Sambhara, David Jacobson, Yvonne Qvarnstrom, Vitaliano Cama

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostic microbiology and infectious disease, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Anna PetersonOak Ridge Institute for Science and Education, Oak Ridge, TN, USA; Centers for Disease Control and Prevention, Global Health Center, Division of Parasitic Diseases and Malaria, Laboratory Science and Diagnostic Branch, Atlanta, GA, USA. Electronic address: yyi6@cdc.gov.
Travis RichinsCenters for Disease Control and Prevention, Global Health Center, Division of Parasitic Diseases and Malaria, Laboratory Science and Diagnostic Branch, Atlanta, GA, USA.
Katelyn HoughtonCenters for Disease Control and Prevention, Global Health Center, Division of Parasitic Diseases and Malaria, Laboratory Science and Diagnostic Branch, Atlanta, GA, USA.
Margarita MishinaCenters for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases, Influenza Division, Immunology and Pathogenesis Branch, Atlanta, GA, USA.
Suresh SharmaCenters for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases, Influenza Division, Immunology and Pathogenesis Branch, Atlanta, GA, USA.
Suryaprakash SambharaCenters for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases, Influenza Division, Immunology and Pathogenesis Branch, Atlanta, GA, USA.
David JacobsonOak Ridge Institute for Science and Education, Oak Ridge, TN, USA; Centers for Disease Control and Prevention, Global Health Center, Division of Parasitic Diseases and Malaria, Laboratory Science and Diagnostic Branch, Atlanta, GA, USA.
Yvonne QvarnstromCenters for Disease Control and Prevention, Global Health Center, Division of Parasitic Diseases and Malaria, Laboratory Science and Diagnostic Branch, Atlanta, GA, USA.
Vitaliano CamaCenters for Disease Control and Prevention, Global Health Center, Division of Parasitic Diseases and Malaria, Laboratory Science and Diagnostic Branch, Atlanta, GA, USA.
Centers for Disease Control and Prevention · USOak Ridge Associated Universities · US

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

Cyclosporiasis is a foodborne diarrheal illness caused by the parasite Cyclospora cayetanensis. The BioFire® FilmArray® gastrointestinal (FilmArray GI) panel is a common method for diagnosing cyclosporiasis from clinical stool samples. The currently published limit of detection (LOD) of this panel is in genome equivalents; however, it is unclear how this relates to the number of C. cayetanensis oocysts in a clinical sample. In this study, we developed a technique to determine the LOD in terms of oocysts, using a cell sorter to sort 1 to 50 C. cayetanensis oocyst(s) previously purified from three human stool sources. We found the FilmArray GI panel detected samples with ≥20 C. cayetanensis oocysts in 100% of replicates, with varying detection among samples with 1, 5, or 10 C. cayetanensis oocysts. This method provides a parasitologically relevant LOD that should enable comparison among C. cayetanensis detection techniques, including the FilmArray GI panel.

Indexed as

CyclosporaCyclosporiasisParasitesAnimalsFecesHumansLimit of DetectionOocystsBioFire FilmArray gastrointestinal panelCyclospora cayetanensisCyclosporiasisOocystParasitic disease

Identifiers

PMID37572510
PMCPMC10530562
OpenAlexW4384937214

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.