Evidence map›Paper›PMID 37572211›Full record

ArticleMolecular biology reports2023

Aflatoxin B1 affects porcine alveolar macrophage growth through the calcium signaling pathway mediated by the ceRNA regulatory network.

Yu Tian, Pei-Yu Dong, Sheng-Lin Liang, Long Li, Shu-Er Zhang, Francesca Gioia Klinger, Wei Shen, You-Yu Yan, Xi-Feng Zhang

Abstract read
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In one paragraph

Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Yu TianSchool of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China.
Pei-Yu DongCollege of Veterinary medicine, Qingdao Agricultural University, Qingdao, 266109, China.
Sheng-Lin LiangCollege of Veterinary medicine, Qingdao Agricultural University, Qingdao, 266109, China.
Long LiCollege of Veterinary medicine, Qingdao Agricultural University, Qingdao, 266109, China.
Shu-Er ZhangAnimal Husbandry General Station of Shandong Province, Jinan, 250010, China.
Francesca Gioia KlingerSaint Camillus International, University of Health Sciences, Via di Sant Alessandro 8, Rome, 00131, Italy.
Wei ShenCollege of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao, 266109, China.
You-Yu YanSchool of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China. yanyy75@163.com.
Xi-Feng ZhangCollege of Veterinary medicine, Qingdao Agricultural University, Qingdao, 266109, China. zhangxf106@qau.edu.cn.
Qingdao Agricultural University · CNWuhan Polytechnic University · CNSaint Camillus International University of Health and Medical Sciences · ITShandong Province Animal Husbandry and Veterinary Bureau · CN

Funding

Gene expression analysis of hormone receptor-negative breast cancer with low HER-2 expression and its potential influence on neoadjuvant chemotherapy 212102310658High level talents research fund project of Qingdao Agricultural University in China 1120043Natural Science Foundation of Shandong Province of China ZR2017MC033Open subject of Hubei Key Laboratory of Animal Nutrition and Feed Science 201913Science & Technology Fund Planning Projects of Qingdao City 21-1-4-ny-7-nshTaishan Scholar Construction Foundation of Shandong province of China ts20190946
6 · The paper itself

Abstract

backgroundAflatoxin B1 (AFB1), one of the most prevalent contaminants in human and animal food, impairs the immune system, but information on the mechanisms of AFB1-mediated macrophage toxicity is still lacking. METHODS AND

resultsIn this study, for the first time, we employed whole transcriptome sequencing technology to explore the molecular mechanism by which AFB1 affects the growth of porcine alveolar macrophages (PAM). We found that AFB1 exposure reduced the proliferative capacity of PAM and prevented cell cycle progression. Based on whole transcriptome analysis, RT-qPCR, ICC and RNAi, we verified the role and regulatory mechanism of the competing endogenous RNA (ceRNA) network in the process of AFB1 exposure affecting the growth of PAM.

conclusionsWe found that AFB1 induced MSTRG.43,583, MSTRG.67,490, MSTRG.84,995, and MSTRG.89,935 to competitively bind miR-219a, miR-30b-3p, and miR-30c-1-3p, eliminating the inhibition of its target genes CACNA1S, RYR3, and PRKCG. This activated the calcium signaling pathway to regulate the growth of PAM. These results provide valuable information on the mechanism of AFB1 exposure induced impairment of macrophage function in humans and animals.

Indexed as

Aflatoxin B1MicroRNAsAnimalsCalcium SignalingHumansMacrophagesMacrophages, AlveolarSwineAflatoxin B1MicroRNAsAflatoxin B1Calcium signaling pathwayceRNAMacrophages

Identifiers

PMID37572211
OpenAlexW4385780846

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.