Evidence map›Paper›PMID 37572210›Full record

ArticleMolecular biology reports2023

Identification of watermelon H3K4 and H3K27 genes and their expression profiles during watermelon fruit development.

Yang Qiao, Yan-Ge Li, Tong-Lu Wei, Hai-Nan Liu, Mao-Song Pei, Xue-Jie Zhu, Zhong-Hou Zhu, Da-Long Guo

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Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Yang QiaoCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China.
Yan-Ge LiLuoyang Nongfa Agricultural Biotechnology Co. Ltd, Luoyang, 471100, Henan Province, China.
Tong-Lu WeiCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China.
Hai-Nan LiuCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China.
Mao-Song PeiCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China.
Xue-Jie ZhuLuoyang Nongfa Agricultural Biotechnology Co. Ltd, Luoyang, 471100, Henan Province, China.
Zhong-Hou ZhuLuoyang Nongfa Agricultural Biotechnology Co. Ltd, Luoyang, 471100, Henan Province, China.
Da-Long GuoCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China. guodalong@haust.edu.cn.

Funding

Joint Research on Agricultural Variety Improvement of Henan Province grant no. 2022010503 to D.L.G.Key Scientific and Technological Projects of Luoyang City (Top of the list) grant no. 2301024A to D.L.G.Program for Innovative Research Team (in Science and Technology) in University of Henan Province grant no. 21IRTSTHN021 to D.L.G.Scientific and technological breakthroughs in Henan Province grant no. 222102110083 to M.S.P.
6 · The paper itself

Abstract

backgroundThe ClaH3K4s and ClaH3K27s gene families are subfamilies of the SET family, each with a highly conserved SET structure domain and a PHD structural domain. Both participate in histone protein methylation, which affects the chromosome structure and gene expression, and is essential for fruit growth and development. METHODS AND

resultsIn order to demonstrate the structure and expression characteristics of ClaH3K4s and ClaH3K27s in watermelon, members of the watermelon H3K4 and H3K27 gene families were identified, and their chromosomal localization, gene structure, and protein structural domains were analyzed. The phylogeny and covariance of the gene families with other species were subsequently determined, and the expression profiles were obtained by performing RNA-Seq and qRT-PCR. The watermelon genome had five H3K4 genes with 3207-8043 bp nucleotide sequence lengths and four H3K27 genes with a 1107-5499 bp nucleotide sequence. Synteny analysis revealed the close relationship between watermelon and cucumber, with the majority of members displaying a one-to-one covariance. Approximately half of the 'Hua-Jing 13 watermelon' ClaH3K4s and ClaH3K27s genes were expressed more in the late fruit development stages, while the changes were minimal for the remaining half. H3K4-2 expression was observed to be slightly greater on day 21 compared to other periods. Moreover, ClaH3K27-1 and ClaH3K27-2 were hardly expressed throughout the developing period, and ClaH3K27-4 exhibited the highest expression.

conclusionThese results serve as a basis for further functional characterization of the H3K4 and H3K27 genes in the fruit development of watermelon.

Indexed as

CitrullusBase SequenceFruitGene Expression Regulation, PlantPhylogenyPolymerase Chain ReactionSyntenyFruit developmentH3K27H3K4Watermelon

Identifiers

PMID37572210

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